Q. Resistance to methotrexate, which inhibits the enzyme dihydrofolate reductase (DHFR) is used as a selectable marker. This enzyme is involved in the synthesis of _____ carbon units and is required for _____ biosynthesis.

View solution

Q. Histidinol dehydrogenase allows synthesis of histidine from exogenous histanol.

View solution

Q. Many mammalian cells contain Thymidine Kinase, the mammalian enzyme uses the analogue _____ than does the viral enzyme.

View solution

Q. A wide range of host cell lines are available and commonly used human cell lines are obtained from ___________

View solution

Q. DEAE-dextran is used for introduction of DNA. It is a modified _____ and is ______

View solution

Q. Cells whose contents have been removed and replaced, by swelling and shrinking in solutions of suitable osmotic strength are called as ____________

View solution

Q. In ___________ cells, virus replication doesn’t take place and viral DNA ___________

View solution

Q. SV40 is a virus and it produces how many transcripts?

View solution

Q. Splicing of the transcripts is necessary for efficient expression.

View solution

Q. T protein is required for replication. It is a product of ____________

View solution

Q. Vaccinia virus is a commonly used virus and it is a member of pox virus family. It has a large genome of _____________ and can accept at least ____________ of foreign DNA.

View solution

Q. Vaccinia virus produces its own ______ which is functional in _____ of infected cells where the virus also replicates.

View solution

Q. It is beneficial to use retrovirus for genetic manipulation of cells in the culture.

View solution

Q. LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.

View solution

Q. The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?

View solution

Q. Choose the incorrect statement in respect to MACs.

View solution

Q. Centromere is the most important component of the human artificial chromosome. It is constructed from tandemly repeated copies of 171 bp of ______ DNA sequence.

View solution

Q. Constitutive promoters are majorly obtained from ________

View solution

Q. In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _____ of tetracycline to the control sequence and _______ transcription.

View solution

Q. Tetracycline binding repressor protein when fused to transcription activator protein from herpes simple virus, then it is called as transactivator protein (tTA).

View solution