Q. Wild-type Drosophila flies are _____ to ethanol supplied in food.

View solution

Q. Cis-acting sites should be present in the vector for ________

View solution

Q. The host should ____ P elements, these elements lead to _______

View solution

Q. Where do P elements integrate into the genome?

View solution

Q. Transient gene silencing can be carried out by microinjecting ______

View solution

Q. Lesch-Nyhan syndrome is caused by the deficiency of the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Cells deficient in HGPRT die in a medium containing which of the following?

View solution

Q. Presence of wild-type DNA onto the HGPRT– in the presence of _______ led to DNA uptake and stable transformation.

View solution

Q. Cells deficient in thymidine kinase (TK) are also killed in HAT medium.

View solution

Q. Hygromycin is used as a selectable marker in mammalian cultured cells. It is used for ____________

View solution

Q. Puromycin is a protein synthesis inhibitor. It is conferred by _______ gene.

View solution

Q. Resistance to bleomycin (zeocin) is used as a selectable marker for mammalian cultured cells and its function is ____________

View solution

Q. Resistance to methotrexate, which inhibits the enzyme dihydrofolate reductase (DHFR) is used as a selectable marker. This enzyme is involved in the synthesis of _____ carbon units and is required for _____ biosynthesis.

View solution

Q. Histidinol dehydrogenase allows synthesis of histidine from exogenous histanol.

View solution

Q. Many mammalian cells contain Thymidine Kinase, the mammalian enzyme uses the analogue _____ than does the viral enzyme.

View solution

Q. A wide range of host cell lines are available and commonly used human cell lines are obtained from ___________

View solution

Q. DEAE-dextran is used for introduction of DNA. It is a modified _____ and is ______

View solution

Q. Cells whose contents have been removed and replaced, by swelling and shrinking in solutions of suitable osmotic strength are called as ____________

View solution

Q. In ___________ cells, virus replication doesn’t take place and viral DNA ___________

View solution

Q. SV40 is a virus and it produces how many transcripts?

View solution

Q. Splicing of the transcripts is necessary for efficient expression.

View solution