Q. Expression of proteins using nuclear polyhedrosis viruses is advantageous because it gives _______ protein yields and post-translational modifications are _________

View solution

Q. The size of the viral genome is large and thus poses a problem. This problem can be resolved by using ____________

View solution

Q. The gene of interest is inserted into transfer vector and then further into viral genome leading to the formation of modified virus. How can plaques from modified virus differentiated from plaques from wild-type virus?

View solution

Q. If the DNA is linearized, what is the effect on the recombination frequency?

View solution

Q. The recombinant virus can be propagated in E.coli and this is referred to as bacmid.

View solution

Q. For the generation of recombinant baculovirus, recombinants can be selected by ____________

View solution

Q. Transgenic Drosophila can be created by microinjection of DNA into the embryos. These embryos are at which stage of development?

View solution

Q. Syncytium is a layer of ______ that have not been separated into individual cells.

View solution

Q. In Drosophila, only nuclei cells give rise to germline cells.

View solution

Q. ______ integration systems are used for the transfer of DNA in Drosophila and it is composed of ______

View solution

Q. Only the _____ part of Drosophila is transgenic and the rest is not. This is known as ______

View solution

Q. rosy gene is used as a selectable marker for transformation in Drosophila, it produces an enzyme required for the synthesis of ____________

View solution

Q. Wild type flies have crimson red eyes whereas rosy mutant have brick-red eyes.

View solution

Q. Wild-type Drosophila flies are _____ to ethanol supplied in food.

View solution

Q. Cis-acting sites should be present in the vector for ________

View solution

Q. The host should ____ P elements, these elements lead to _______

View solution

Q. Where do P elements integrate into the genome?

View solution

Q. Transient gene silencing can be carried out by microinjecting ______

View solution

Q. Lesch-Nyhan syndrome is caused by the deficiency of the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Cells deficient in HGPRT die in a medium containing which of the following?

View solution

Q. Presence of wild-type DNA onto the HGPRT– in the presence of _______ led to DNA uptake and stable transformation.

View solution