Q. Autographa californica nuclear polyhedrois virus (AcNPV) is commonly used bacilovirus for infecting cultured cells. The virus is ________ and _______

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Q. DNA replication of the AcNPV takes 6 hours after infection. After how many hours of infection new virus particles are produced by budding?

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Q. The virus particles are held together by polyhedron and p10 protein.

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Q. The polyhedrin and p10 protein are produced in ____________ amount and other genes are expressed in _________ amount.

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Q. Expression of proteins using nuclear polyhedrosis viruses is advantageous because it gives _______ protein yields and post-translational modifications are _________

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Q. The size of the viral genome is large and thus poses a problem. This problem can be resolved by using ____________

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Q. The gene of interest is inserted into transfer vector and then further into viral genome leading to the formation of modified virus. How can plaques from modified virus differentiated from plaques from wild-type virus?

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Q. If the DNA is linearized, what is the effect on the recombination frequency?

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Q. The recombinant virus can be propagated in E.coli and this is referred to as bacmid.

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Q. For the generation of recombinant baculovirus, recombinants can be selected by ____________

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Q. Transgenic Drosophila can be created by microinjection of DNA into the embryos. These embryos are at which stage of development?

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Q. Syncytium is a layer of ______ that have not been separated into individual cells.

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Q. In Drosophila, only nuclei cells give rise to germline cells.

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Q. ______ integration systems are used for the transfer of DNA in Drosophila and it is composed of ______

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Q. Only the _____ part of Drosophila is transgenic and the rest is not. This is known as ______

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Q. rosy gene is used as a selectable marker for transformation in Drosophila, it produces an enzyme required for the synthesis of ____________

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Q. Wild type flies have crimson red eyes whereas rosy mutant have brick-red eyes.

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Q. Wild-type Drosophila flies are _____ to ethanol supplied in food.

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Q. Cis-acting sites should be present in the vector for ________

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Q. The host should ____ P elements, these elements lead to _______

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