Recombinant DNA Technology MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Recombinant DNA Technology, a fundamental topic in the field of Biochemistry. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Recombinant DNA Technology MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Recombinant DNA Technology mcq questions that explore various aspects of Recombinant DNA Technology problems. Each MCQ is crafted to challenge your understanding of Recombinant DNA Technology principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biochemistry tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Recombinant DNA Technology MCQs are your pathway to success in mastering this essential Biochemistry topic.

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Recombinant DNA Technology MCQs | Page 13 of 21

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Q121.
Which of the following does not act as a restriction enzyme?

a.

EcorI

b.

BamHI

c.

HindIII

d.

polydeoxyribonucleotide synthase

Discuss
Answer: (d).polydeoxyribonucleotide synthase
Q122.
Which of the following enzyme is responsible for making a DNA copy from RNA?

a.

Reverse transcriptase

b.

DNA polymerase

c.

RNA polI

d.

RNA polII

Discuss
Answer: (a).Reverse transcriptase
Q123.
Which of the following statements is true?

a.

Exonuclease III removes nucleotide residues from the 3’ ends of a DNA strand

b.

Bacteriophage lambda exonuclease removes terminal phosphates

c.

Alkaline phosphatase removes nucleotides from 5‘ends

d.

Kinase adds homopolymer tails to the 3’ –OH ends of a linear duplex

Discuss
Answer: (a).Exonuclease III removes nucleotide residues from the 3’ ends of a DNA strand
Q124.
Which of the following can be termed as a restriction modification system?

a.

Restriction endonuclease + methylase

b.

DNA ligase + methylase

c.

Restriction endonuclease + acetylase

d.

DNA ligase + acetylase

Discuss
Answer: (a).Restriction endonuclease + methylase
Q125.
Which of the following is true about restriction endonucleases?

a.

Type I and II requires ATP to move along DNA

b.

Type I, II and III requires ATP to move along DNA

c.

Type II requires no ATP and cleaves DNA within recognition sequence

d.

Type II requires ATP and cleaves DNA within recognition sequence

Discuss
Answer: (c).Type II requires no ATP and cleaves DNA within recognition sequence
Q126.
Which of the following enzymes’ combined action leads to the generation of sticky ends in the plasmid vector?

a.

Alkaline phosphatase and terminal transferase

b.

Exonuclease III and alkaline phosphatase

c.

Bacteriophage lambda exonuclease and terminal transferase

d.

Exonuclease III and terminal transferase

Discuss
Answer: (c).Bacteriophage lambda exonuclease and terminal transferase
Q127.
Generally a plasmid vector contains how many elements?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (c).3
Q128.
To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?

a.

MgCl₂

b.

CaCl₂

c.

NaCl

d.

HCl

Discuss
Answer: (b).CaCl₂
Q129.
Which of the following statements is false?

a.

The cells which take up recombinant plasmids are drug resistant

b.

The cells with no recombinant plasmids are drug resistant

c.

Plasmid replication is independent of cell’s division

d.

The cells containing recombinant plasmid are called transformed cells

Discuss
Answer: (a).The cells which take up recombinant plasmids are drug resistant
Q130.
The extra chromosomal, self-replicating, closed, double stranded and circular DNA molecule is generally termed as __________

a.

Chromosome

b.

Plasmid

c.

Genomic DNA

d.

Bacteriophage

Discuss
Answer: (b).Plasmid
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