Recombinant DNA Technology MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Recombinant DNA Technology, a fundamental topic in the field of Biochemistry. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Recombinant DNA Technology MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Recombinant DNA Technology mcq questions that explore various aspects of Recombinant DNA Technology problems. Each MCQ is crafted to challenge your understanding of Recombinant DNA Technology principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biochemistry tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Recombinant DNA Technology MCQs are your pathway to success in mastering this essential Biochemistry topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Recombinant DNA Technology. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

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Recombinant DNA Technology MCQs | Page 13 of 21

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Q121.
Which of the following endonuclease removes a length of DNA between two telomere sequences?

a.

EcoR1

b.

EcoR2

c.

BamHI

d.

HindIII

Discuss
Answer: (c).BamHI
Q122.
In hybridization method, which of the following is pressed onto agar?

a.

Silicon paper

b.

Nitrocellulose paper

c.

Filter paper

d.

PVDF

Discuss
Answer: (b).Nitrocellulose paper
Q123.
Which of the following temperature best suits for the growth and multiplication of transformed cells?

a.

35°C

b.

36°C

c.

37°C

d.

30°C

Discuss
Answer: (c).37°C
Q124.
Which of the following is a DNA element that instructs RNA polymerase where to bind on DNA?

a.

Operator

b.

Promoter

c.

Enhancer

d.

Hormone response element

Discuss
Answer: (b).Promoter
Q125.
Site directed mutagenesis facilitated research on __________

a.

Carbohydrates

b.

Proteins

c.

Lipids

d.

Fats

Discuss
Answer: (b).Proteins
Q126.
Recombinant DNA molecule is produced by ligating __________

a.

Two DNA segments

b.

Two m-RNA molecules

c.

One m-RNA with one t-RNA

d.

One m-RNA with DNA segment

Discuss
Answer: (a).Two DNA segments
Q127.
Arrange the following steps involved in Gene cloning in the correct order.

i) Isolation of desired gene
ii) Insertion of isolated gene to the vector
iii) Introduction of recombinant vector to the host
iv) Expression of recombinant gene in host
v) Extraction of recombinant gene product

a.

i, ii, iii, iv, v

b.

v, iv, iii, ii, i

c.

i, iii, ii, iv, v

d.

iv, v, ii, i, iii

Discuss
Answer: (a).i, ii, iii, iv, v
Q128.
Where does a restriction endonuclease cuts DNA?

a.

Ligation site

b.

Recognition sequence

c.

Ori

d.

Replication site

Discuss
Answer: (b).Recognition sequence
Q129.
Which of the following statements is true about a vector?

a.

Plasmids, phages can be used as vectors

b.

All vectors are plasmids

c.

Cyanobacteria can also be used as vectors

d.

Fungi can also be used as vectors

Discuss
Answer: (a).Plasmids, phages can be used as vectors
Q130.
Which of the following is true about plasmids?

a.

They are present in all organisms

b.

They are present in bacteria only

c.

They are present in bacteria and phages

d.

They are present in plants and animals

Discuss
Answer: (c).They are present in bacteria and phages
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