Recombinant DNA Technology MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Recombinant DNA Technology, a fundamental topic in the field of Biochemistry. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Recombinant DNA Technology MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Recombinant DNA Technology mcq questions that explore various aspects of Recombinant DNA Technology problems. Each MCQ is crafted to challenge your understanding of Recombinant DNA Technology principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biochemistry tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Recombinant DNA Technology MCQs are your pathway to success in mastering this essential Biochemistry topic.

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Recombinant DNA Technology MCQs | Page 10 of 21

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Q91.
Which of the following is correct in terms of determination of location of genetic traits?

a.

Known protein coding sequences are too far apart to allow linkage determination for most new genes

b.

Restriction sites allow DNAs to be digested

c.

Protein-coding genes are always associated with a restriction pattern

d.

None of the above

Discuss
Answer: (a).Known protein coding sequences are too far apart to allow linkage determination for most new genes
Q92.
In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel.

a.

negative ... positive

b.

basic ... acidic

c.

long ... short

d.

positive to negative

Discuss
Answer: (a).negative ... positive
Q93.
Restriction enzymes

a.

protect bacteria from viral infection

b.

cut DNA in a staggered fashion

c.

cut DNAs producing a blunt end

d.

all of the above

Discuss
Answer: (d).all of the above
Q94.
First discovered, Type II restriction endonuclease was

a.

Hinf I

b.

Eco K

c.

Hind II

d.

EcoRI

Discuss
Answer: (c).Hind II
Q95.
Which of the following techniques can be used to determine the defective gene and for developing cancer?

a.

Western blot

b.

Southern blot

c.

Northern blot

d.

Eastern blot

Discuss
Answer: (b).Southern blot
Q96.
The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ .

a.

would be of no concern if it occurred.

b.

has occurred

c.

can never occur

d.

seems unlikely

Discuss
Answer: (d).seems unlikely
Q97.
Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because

a.

in both cases molecules migrate to the anode

b.

both techniques rely on a constant charge to mass ratio

c.

both techniques utilize the sieving properties of gels

d.

all of the above

Discuss
Answer: (d).all of the above
Q98.
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

a.

copies of the gene with an altered sequence so that a restriction site is inserted

b.

mutagenized copies of a gene

c.

short sequences that will hybridize only to a specific base sequence

d.

PCR-amplified variable numbers of tandem repeats (VNTRs)

Discuss
Answer: (c).short sequences that will hybridize only to a specific base sequence
Q99.
In order to insert a foreign gene into a plasmid, both must __________

a.

have identical DNA sequences

b.

originate from the same type of cell

c.

be cut by the same restriction enzyme

d.

be of the same length

Discuss
Answer: (c).be cut by the same restriction enzyme
Q100.
Plant transformation can be obtained by

a.

combining plant and animal cells in culture

b.

shooting DNA into plant cells with a gun

c.

using the E. coli bacterium to infect plant roots

d.

infecting plants with a tobacco mosaic virus

Discuss
Answer: (b).shooting DNA into plant cells with a gun
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