Recombinant DNA Technology MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Recombinant DNA Technology, a fundamental topic in the field of Biochemistry. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Recombinant DNA Technology MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Recombinant DNA Technology mcq questions that explore various aspects of Recombinant DNA Technology problems. Each MCQ is crafted to challenge your understanding of Recombinant DNA Technology principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biochemistry tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Recombinant DNA Technology MCQs are your pathway to success in mastering this essential Biochemistry topic.

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Recombinant DNA Technology MCQs | Page 9 of 21

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Q81.
An example of a restriction fragment length polymorphism is

a.

an Eco RI cuts DNA at a different sequence than Hind III

b.

different length fragments of DNA resulting from loss or gain of a restriction site

c.

cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved

d.

all of the above

Discuss
Answer: (b).different length fragments of DNA resulting from loss or gain of a restriction site
Q82.
Knockout mice are created by

a.

mutagenizing a mouse and selecting for mutant offspring

b.

creating a chimera by fusing cells from two different cell lines

c.

infecting the mouse with a retrovirus

d.

transfecting embryonic stem cells with an altered gene sequence

Discuss
Answer: (d).transfecting embryonic stem cells with an altered gene sequence
Q83.
A plasmid

a.

is a circular DNA molecule

b.

always contains an origin of replication

c.

usually contains one or more restriction sites

d.

all of the above

Discuss
Answer: (d).all of the above
Q84.
Under which of the following conditions would population gene frequencies remain the same?

a.

Selection for homozygotes

b.

Small population size

c.

Active migration between groups

d.

Random mating

Discuss
Answer: (d).Random mating
Q85.
Which of the following genetic diseases would be amenable to genetic engineering?

a.

Down's syndrome

b.

Muscular dystrophy

c.

Cystic fibrosis

d.

Cri du Chat

Discuss
Answer: (c).Cystic fibrosis
Q86.
X-rays cause

a.

the formation of thymine dimers

b.

ionization of water in the cell

c.

heat

d.

none of the above

Discuss
Answer: (b).ionization of water in the cell
Q87.
The order for the construction of a cDNA fragment from mRNA is to

a.

bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

b.

treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT

c.

digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase

d.

bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase

Discuss
Answer: (a).bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
Q88.
The TP53 gene of chromosome 17 codes for a protein __________ .

a.

that plays a role in the digestive process

b.

involved in glucose transport

c.

involved in the regulation of the cell cycle

d.

that is like a white blood cell protein

Discuss
Answer: (c).involved in the regulation of the cell cycle
Q89.
Why is golden rice pale yellow in color?

a.

It is rich in chlorophyll a.

b.

It is rich in beta-carotene.

c.

It is rich in chlorophyll b.

d.

It is rich in phycobilins.

Discuss
Answer: (b).It is rich in beta-carotene.
Q90.
When populations are small, gene frequencies can change from generation to generation and some alleles may become fixed in a population. This is called __________ .

a.

assortative mating

b.

inbreeding

c.

heterosis

d.

genetic drift

Discuss
Answer: (d).genetic drift
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