Spectroscopy and Chromatography MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Spectroscopy and Chromatography, a fundamental topic in the field of Biochemistry. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Spectroscopy and Chromatography MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Spectroscopy and Chromatography mcq questions that explore various aspects of Spectroscopy and Chromatography problems. Each MCQ is crafted to challenge your understanding of Spectroscopy and Chromatography principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biochemistry tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Spectroscopy and Chromatography MCQs are your pathway to success in mastering this essential Biochemistry topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Spectroscopy and Chromatography. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Spectroscopy and Chromatography knowledge to the test? Let's get started with our carefully curated MCQs!

Spectroscopy and Chromatography MCQs | Page 1 of 10

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Q1.
Which of the following components of a monochromator is the dispersing element?

a.

The collimating lens

b.

The entrance slit

c.

The diffraction grating

d.

None of these

Discuss
Answer: (c).The diffraction grating
Q2.
A simple harmonic oscillator may absorb energy

a.

at anytime.

b.

when the frequencies match exactly

c.

when the amplitudes are the same.

d.

at no time.

Discuss
Answer: (b).when the frequencies match exactly
Q3.
UV-Vis spectroscopy of organic compounds is usually concerned with which electronic transition(s)?

a.

ฯƒ โ†’ ฯƒ*

b.

n โ†’ ฯƒ*

c.

n โ†’ ฯ€* and ฯ€ โ†’ ฯ€*

d.

none of these

Discuss
Answer: (c).n โ†’ ฯ€* and ฯ€ โ†’ ฯ€*
Q4.
Vibrational spectroscopy is

a.

a large mass on a weak spring

b.

a flashlight through a prism and shake it

c.

a class of spectroscopic techniques which analyzes molecular motions

d.

an Infrared spectroscopy

Discuss
Answer: (c).a class of spectroscopic techniques which analyzes molecular motions
Q5.
Why are rotational transitions of little use to a spectroscopist?

a.

Because the energy required to induce a rotational transition is so small that it cannot be measured

b.

Because rotational transitions are extremely rare

c.

Because, in liquids and solids, spectral lines corresponding to rotational transitions are broadened as the result of molecular collisions and other interactions

d.

All of the above

Discuss
Answer: (c).Because, in liquids and solids, spectral lines corresponding to rotational transitions are broadened as the result of molecular collisions and other interactions
Q6.
Beer's Law states that

a.

absorbance is proportional to both the path length and concentration of the absorbing species

b.

absorbance is proportional to the log of the concentration of the absorbing species

c.

absorbance is equal to P0 / P

d.

none of the above

Discuss
Answer: (a).absorbance is proportional to both the path length and concentration of the absorbing species
Q7.
Molar absorbtivities of compounds exhibiting charge transfer absorption are

a.

small

b.

moderate

c.

large

d.

none of these

Discuss
Answer: (c).large
Q8.
Molar absorbtivity is the measure of the

a.

amount of light absorbed per unit length

b.

amount of light absorbed per unit concentration

c.

amount of light reflected and absorbed per unit concentration

d.

None of the above

Discuss
Answer: (b).amount of light absorbed per unit concentration
Q9.
Which of the following relationships between absorbance and %transmittance is incorrect?

a.

A = log10 100 / %T

b.

A = 2 - log10 %T

c.

A = log10 1 / T

d.

All are correct

Discuss
Answer: (c).A = log10 1 / T
Q10.
Why is it generally preferable to use absorbance as a measure of absorption rather than % transmittance?

a.

Because %T cannot be measured as accurately as absorbance

b.

Because %T is dependant on the power of the incident radiation

c.

Because absorbance is proportional to the concentration of the analyte, whereas %T is not

d.

none of the above

Discuss
Answer: (c).Because absorbance is proportional to the concentration of the analyte, whereas %T is not
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