Spectroscopy and Chromatography MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Spectroscopy and Chromatography, a fundamental topic in the field of Biochemistry. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Spectroscopy and Chromatography MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Spectroscopy and Chromatography mcq questions that explore various aspects of Spectroscopy and Chromatography problems. Each MCQ is crafted to challenge your understanding of Spectroscopy and Chromatography principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biochemistry tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Spectroscopy and Chromatography MCQs are your pathway to success in mastering this essential Biochemistry topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Spectroscopy and Chromatography. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Spectroscopy and Chromatography knowledge to the test? Let's get started with our carefully curated MCQs!

Spectroscopy and Chromatography MCQs | Page 2 of 10

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Q11.
In the past, IR spectra had to be aquired one wavelength at a time, which took a long time. Today quick spectra is due to the

a.

the Fourier Transfer Algorithm allows us to scan all frequencies at once

b.

light is faster today that it used to be

c.

absence of broad spectrum of wavelenth

d.

none of the above

Discuss
Answer: (a).the Fourier Transfer Algorithm allows us to scan all frequencies at once
Q12.
Which of the following is not an IR vibrational mode?

a.

Stretching

b.

Scissoring

c.

Rocking

d.

Rolling

Discuss
Answer: (d).Rolling
Q13.
Which of the following will oscillate the fastest?

a.

A large mass on a weak spring

b.

A large mass on a stiff spring

c.

A small mass on a stiff spring

d.

A small mass on a weak spring

Discuss
Answer: (c).A small mass on a stiff spring
Q14.
In the equation, A = ε bc, what quantity is represented by "ε"?

a.

Absorbtivity

b.

Molar absorbtivity

c.

Path length

d.

None of these

Discuss
Answer: (b).Molar absorbtivity
Q15.
Why must the voltage supplied to a tungsten lamp be very stable?

a.

Because if it wasn't, the lamp would bum out

b.

Because amount of energy the lamp emits is proportional to the fourth power of the operating voltage

c.

Because the lamp will only function at a specific voltage

d.

All of the above

Discuss
Answer: (b).Because amount of energy the lamp emits is proportional to the fourth power of the operating voltage
Q16.
Where does a carbonyl (C=0) stretch appear in an IR spectrum?

a.

1740-1720

b.

1870-1650

c.

3640-3250

d.

160-110

Discuss
Answer: (b).1870-1650
Q17.
What is the relationship between wavelength and wave number?

a.

Wavenumber = 1 / wavelength in centimeters

b.

Wavenumber - wavelength in nanometers = 1

c.

Wavelength in nanometers x wavenumber = 1

d.

None of the above

Discuss
Answer: (a).Wavenumber = 1 / wavelength in centimeters
Q18.
In the intersystem crossing

a.

the spin of an excited electron reverses, changing the state of the molecule (from singlet state to triplet state or vice versa)

b.

a molecule converts its excess energy to light, and emits a photon

c.

a molecule converts excess electronic energy to vibrational energy

d.

all of the above

Discuss
Answer: (a).the spin of an excited electron reverses, changing the state of the molecule (from singlet state to triplet state or vice versa)
Q19.
Why must the radiation source for fluorescence spectrometry be more powerful than for absorption spectroscopy?

a.

Because the magnitude of the output signal is proportional to the power of the incident radiation

b.

Because the sample won't fluoresce if the incident radiation is of low power

c.

To allow for scattering by the sample

d.

None of the above

Discuss
Answer: (a).Because the magnitude of the output signal is proportional to the power of the incident radiation
Q20.
Why do fluorescence spectrometers often use double-beam optics?

a.

So a reference solution can be used

b.

To compensate for beam attenuation by the monochromator

c.

To compensate for power fluctuations in the radiation source

d.

All of the above

Discuss
Answer: (c).To compensate for power fluctuations in the radiation source
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