Spectroscopy and Chromatography MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Spectroscopy and Chromatography, a fundamental topic in the field of Biochemistry. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Spectroscopy and Chromatography MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Spectroscopy and Chromatography mcq questions that explore various aspects of Spectroscopy and Chromatography problems. Each MCQ is crafted to challenge your understanding of Spectroscopy and Chromatography principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biochemistry tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Spectroscopy and Chromatography MCQs are your pathway to success in mastering this essential Biochemistry topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Spectroscopy and Chromatography. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Spectroscopy and Chromatography knowledge to the test? Let's get started with our carefully curated MCQs!

Spectroscopy and Chromatography MCQs | Page 7 of 10

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Q61.
An FT-IR instrument record a signal in the

a.

time domain

b.

frequency domain

c.

both (a) and (b)

d.

none of these

Discuss
Answer: (a).time domain
Q62.
When radiation energy is absorbed by a spin 1/2 nucleus in a magnetic field, what happens?

a.

The processional frequency of the nucleus increases

b.

The nucleus spins faster

c.

The angle of precession flips so that the magnetic moment of the nucleus opposes the applied field

d.

none of the above

Discuss
Answer: (c).The angle of precession flips so that the magnetic moment of the nucleus opposes the applied field
Q63.
Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis?

a.

Molecular mass

b.

Solubility

c.

Isoelectric point

d.

Folding

Discuss
Answer: (c).Isoelectric point
Q64.
What is meant by rotating frame of reference?

a.

That the sample is spun rapidly in the applied field

b.

If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space

c.

That the detector rotates around the sample

d.

None of the above

Discuss
Answer: (b).If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space
Q65.
The sequence of amino acids in proteins can be determined by means of

a.

identification of the -NH₂ terminal amino acids

b.

identification of the -COOH terminal amino acids

c.

partial cleavage of the original polypeptide into smaller polypeptides

d.

all of the above

Discuss
Answer: (d).all of the above
Q66.
Why is it advantageous to record many FID signals from the same sample and then add them together?

a.

To ensure that all target nuclei in the sample have been excited

b.

To remove inaccuracies caused by fluctuations in the applied magnetic field

c.

To increase sensitivity

d.

None of the above

Discuss
Answer: (c).To increase sensitivity
Q67.
In FT-NMR, how are nuclei excited?

a.

By radio-frequency radiation whose frequency is swept across a predetermined range

b.

By an intense pulse of radiation which contains a wide range of frequencies

c.

By an intense pressure

d.

None of the above

Discuss
Answer: (a).By radio-frequency radiation whose frequency is swept across a predetermined range
Q68.
Negative staining is a technique used in

a.

electron microscopy

b.

gel electrophoresis

c.

immunocytochemistry

d.

light microscopy

Discuss
Answer: (a).electron microscopy
Q69.
What is the typical internal diameter of fused silica capillary columns?

a.

0.2-0.3 mm

b.

0.3-0.5mm

c.

0.5-1.0 mm

d.

1.0-2.0 mm

Discuss
Answer: (a).0.2-0.3 mm
Q70.
Resolution is proportional to the

a.

number of theoretical plates in a column

b.

square root of the number of theoretical plates in a column

c.

square of the number of theoretical plates in a column

d.

cube root of the number of theoretical plates in a column

Discuss
Answer: (b).square root of the number of theoretical plates in a column
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