Spectroscopy and Chromatography MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Spectroscopy and Chromatography, a fundamental topic in the field of Biochemistry. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Spectroscopy and Chromatography MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Spectroscopy and Chromatography mcq questions that explore various aspects of Spectroscopy and Chromatography problems. Each MCQ is crafted to challenge your understanding of Spectroscopy and Chromatography principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biochemistry tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Spectroscopy and Chromatography MCQs are your pathway to success in mastering this essential Biochemistry topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Spectroscopy and Chromatography. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Spectroscopy and Chromatography knowledge to the test? Let's get started with our carefully curated MCQs!

Spectroscopy and Chromatography MCQs | Page 9 of 10

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Q81.
Column bleeding occurs when

a.

elution of the analyte is extended over time

b.

the column is cracked and stationary phase leaks out

c.

traces of the stationary phase are eluted

d.

the column breaks during installation and causes personal injury

Discuss
Answer: (c).traces of the stationary phase are eluted
Q82.
Which of the following are not used as stationary phases in a GC column?

a.

Polysiloxanes

b.

Silica

c.

Cyclodextrins

d.

None are used as stationary phases

Discuss
Answer: (b).Silica
Q83.
Doubling the column's length increases resolution by a factor of

a.

(2)^0.5

b.

2

c.

3

d.

4

Discuss
Answer: (a).(2)^0.5
Q84.
Sample injection is considered successful if

a.

all of the sample in the injector has been added to the column

b.

the sample is concentrated at the start of the column

c.

the sample is spread evenly along the column

d.

the sample is homogenously spread along the column

Discuss
Answer: (b).the sample is concentrated at the start of the column
Q85.
Theoretical plates are used to

a.

estimate the efficiency of a column

b.

determine the thickness of the stationary phase

c.

measure the distribution of the analyte between mobile and stationary phases

d.

None of the above

Discuss
Answer: (a).estimate the efficiency of a column
Q86.
What does the selectivity factor describe?

a.

The proportional difference in widths of two chromatographic peaks

b.

The maximum number of different species which a column can separate simultaneously

c.

The relative separation achieved between two species

d.

None of the above

Discuss
Answer: (c).The relative separation achieved between two species
Q87.
Helium is generally preferred as carrier gas over nitrogen and hydrogen because

a.

it is inert

b.

it has a lower viscosity

c.

it doubles up as a party gas for balloons and funny voices

d.

all of above

Discuss
Answer: (d).all of above
Q88.
The column is heated to

a.

prevent analyte condensation within the column

b.

control elution of the different analytes

c.

reduce band broadening to get sharper peaks

d.

all of these

Discuss
Answer: (d).all of these
Q89.
What are the benefits of decreasing the column internal diameter?

a.

Increased sample capacity

b.

Increased resolution

c.

Reduced risk of column overloading

d.

All of the above

Discuss
Answer: (b).Increased resolution
Q90.
A retention gap is placed between the injector and the front of the column to

a.

retain contaminants and prevent them from reaching the column

b.

retain the sample and release it gradually to the column

c.

prevent backflush of the injected solution

d.

all of the above

Discuss
Answer: (a).retain contaminants and prevent them from reaching the column
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