Spectroscopy and Chromatography MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Spectroscopy and Chromatography, a fundamental topic in the field of Biochemistry. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Spectroscopy and Chromatography MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Spectroscopy and Chromatography mcq questions that explore various aspects of Spectroscopy and Chromatography problems. Each MCQ is crafted to challenge your understanding of Spectroscopy and Chromatography principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biochemistry tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Spectroscopy and Chromatography MCQs are your pathway to success in mastering this essential Biochemistry topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Spectroscopy and Chromatography. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Spectroscopy and Chromatography knowledge to the test? Let's get started with our carefully curated MCQs!

Spectroscopy and Chromatography MCQs | Page 3 of 10

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Q21.
For a molecule to absorb IR, why must the molecule's vibrations cause fluctuations in the dipole moment of the molecule?

a.

Because a change in dipole moment lowers the energy required for electronic transitions

b.

Because for absorption to occur, the radiation must interact with the electric field caused by changing dipole moment

c.

Because fluctuations in the dipole moment allow the molecule to deform by bending and stretching

d.

All of the above

Discuss
Answer: (b).Because for absorption to occur, the radiation must interact with the electric field caused by changing dipole moment
Q22.
Internal conversion is where

a.

the spin of an excited electron reverses, changing the state of the molecule (from singlet state to triplet state or vice versa)

b.

A molecule converts its excess energy to light, and emits a photon

c.

A molecule converts excess electronic energy to vibrational energy

d.

none of the above

Discuss
Answer: (c).A molecule converts excess electronic energy to vibrational energy
Q23.
Why phosphorescence measurements are usually made at a low temperature?

a.

To prevent thermal degradation of the phosphorescent species

b.

To promote phosphorescence by slowing the rate of radiationless transfer processes

c.

To increase the efficiency of the detector

d.

To decease the efficiency of detector

Discuss
Answer: (b).To promote phosphorescence by slowing the rate of radiationless transfer processes
Q24.
Fluorescence occurs when

a.

a molecule returns to the electronic ground state from an excited triplet state by losing it's excess energy as a photon

b.

a molecule returns to the electronic ground state from an excited singlet state by losing it's excess energy as a photon

c.

a molecule lowers its vibrational energy by losing it's excess energy as a photon

d.

none of the above

Discuss
Answer: (b).a molecule returns to the electronic ground state from an excited singlet state by losing it's excess energy as a photon
Q25.
Thin layer chromatography is

a.

partition chromatography

b.

electrical mobility of ionic species

c.

adsorption chromatography

d.

none of the above

Discuss
Answer: (c).adsorption chromatography
Q26.
In gas chromatography, the basis for separation of the components of the volatile material is the difference in

a.

partition coefficients

b.

conductivity

c.

molecular weight

d.

molarity

Discuss
Answer: (a).partition coefficients
Q27.
In reverse phase chromatography, the stationary phase is made

a.

non-polar

b.

polar

c.

either non-polar or polar

d.

none of these

Discuss
Answer: (a).non-polar
Q28.
Ion exchange chromatography is based on the

a.

electrostatic attraction

b.

electrical mobility of ionic species

c.

adsorption chromatography

d.

partition chromatography

Discuss
Answer: (a).electrostatic attraction
Q29.
The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)

a.

V = V0 + kDVi

b.

V = V0/Vi

c.

V = V0 - kDVi

d.

V/V0 = kDVi

Discuss
Answer: (a).V = V0 + kDVi
Q30.
A combination of paper chromatography and electrophoresis involves

a.

partition chromatography

b.

electrical mobility of the ionic species

c.

both (a) and (b)

d.

none of these

Discuss
Answer: (c).both (a) and (b)
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