Spectroscopy and Chromatography MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Spectroscopy and Chromatography, a fundamental topic in the field of Biochemistry. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Spectroscopy and Chromatography MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Spectroscopy and Chromatography mcq questions that explore various aspects of Spectroscopy and Chromatography problems. Each MCQ is crafted to challenge your understanding of Spectroscopy and Chromatography principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biochemistry tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Spectroscopy and Chromatography MCQs are your pathway to success in mastering this essential Biochemistry topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Spectroscopy and Chromatography. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Spectroscopy and Chromatography knowledge to the test? Let's get started with our carefully curated MCQs!

Spectroscopy and Chromatography MCQs | Page 5 of 10

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Q41.
Dwell volume is defined as

a.

the volume of solvent contained in a liquid chromatographic column

b.

the time required for the gradient to reach the column

c.

the volume of the column between the point at which solvents are mixed and the beginning of the column

d.

none of these

Discuss
Answer: (c).the volume of the column between the point at which solvents are mixed and the beginning of the column
Q42.
Void volume refers to the

a.

total volume of eluent in the column the remainder being taken up by the packing material

b.

the volume of solvent contained in a liquid chromatographic column

c.

the time required for the gradient to reach the column

d.

the volume of the column between the point at which solvents are mixed and the beginning of the column

Discuss
Answer: (a).total volume of eluent in the column the remainder being taken up by the packing material
Q43.
In normal phase HPLC, there is a

a.

non polar solvent/polar column

b.

polar solvent/non-polar column

c.

non polar solvent/non-polar column

d.

any of the above

Discuss
Answer: (a).non polar solvent/polar column
Q44.
Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)?

a.

Greater sensitivity and reusable columns

b.

Ideal for ionic species and large molecules

c.

Sample recovery

d.

All of the above

Discuss
Answer: (d).All of the above
Q45.
Which can be used as a mobile phase in HPLC applications?

a.

Any compound with solubility in liquid

b.

Any compound with limited solubility in liquid

c.

Any compound with non-solubility in liquid

d.

Any of the above

Discuss
Answer: (a).Any compound with solubility in liquid
Q46.
What is shielding in NMR?

a.

Using a curved piece of metal to block an opponents attack

b.

Putting metal around an Rf source

c.

When the magnetic moment of an atom blocks the full induced magnetic field from surrounding nuclei

d.

Blocking parts of a molecule from Rf radiation

Discuss
Answer: (c).When the magnetic moment of an atom blocks the full induced magnetic field from surrounding nuclei
Q47.
What is used to cool the superconducting coil?

a.

Hydrogen

b.

Ice

c.

Dry ice

d.

Liquid helium

Discuss
Answer: (d).Liquid helium
Q48.
Coupling causes the peaks in 1H NMR spectra to be split into

a.

two peaks

b.

multiple peaks equal to the number of hydrogens on surrounding atoms

c.

multiple peaks equal to the number of surrounding carbon atoms

d.

multiple peaks equal to the number of hydrogen on surrounding atoms, plus one

Discuss
Answer: (d).multiple peaks equal to the number of hydrogen on surrounding atoms, plus one
Q49.
When placed in a magnetic field, all the random spins of the nuclei

a.

stop

b.

reverse direction

c.

align with the magnetic field

d.

rotate to 90° away from the induced field

Discuss
Answer: (c).align with the magnetic field
Q50.
Better understanding of the nuclei is possible,

a.

with the help of wavelength spectrum

b.

with the help of frequencies ranges

c.

with the help of a mathematical translator called the fourier transfer algorithm

d.

none of the above

Discuss
Answer: (c).with the help of a mathematical translator called the fourier transfer algorithm
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