Recombinant DNA Technology MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Recombinant DNA Technology, a fundamental topic in the field of Biochemistry. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Recombinant DNA Technology MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Recombinant DNA Technology mcq questions that explore various aspects of Recombinant DNA Technology problems. Each MCQ is crafted to challenge your understanding of Recombinant DNA Technology principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biochemistry tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Recombinant DNA Technology MCQs are your pathway to success in mastering this essential Biochemistry topic.

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Recombinant DNA Technology MCQs | Page 6 of 21

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Q51.
The major function of RNA polymerase's sigma factor is

a.

recognition of the translational stop sequence

b.

recognition of the transcriptional start sequence

c.

recognition of the transcriptional stop sequence

d.

recognition of the translational start sequence

Discuss
Answer: (b).recognition of the transcriptional start sequence
Q52.
Most transcripts of protein coding genes are cleaved

a.

at the 3' end just after a poly adenylation signal

b.

at the 3' end just before a poly adenylation signal

c.

at the 5' end immediately after a poly adenylation signal

d.

at the 5' end immediately before a poly adenylation signal

Discuss
Answer: (a).at the 3' end just after a poly adenylation signal
Q53.
RNA is synthesized on a DNA template in a process called __________ , which utilizes the enzyme __________

a.

translation, RNA polymerase

b.

transcription, DNA polymerase

c.

transcription, RNA polymerase

d.

replication, DNA polymerase

Discuss
Answer: (c).transcription, RNA polymerase
Q54.
The closed complex at bacterial promoters is

a.

in equilibrium with free RNA polymerase and the promoter

b.

not affected by promoter mutations

c.

bound more tightly when repressors are present

d.

not affected by activator proteins

Discuss
Answer: (a).in equilibrium with free RNA polymerase and the promoter
Q55.
Transcription begins

a.

at the start codon

b.

upstream from the start codon

c.

downstream from the start codon

d.

none of these

Discuss
Answer: (b).upstream from the start codon
Q56.
Transcription results in

a.

an amino acid chain

b.

messenger RNA

c.

complementary DNA

d.

Okazaki fragments

Discuss
Answer: (b).messenger RNA
Q57.
RNA polymerase holoenzyme initiates transcription which involves

a.

recognition of the -10 sequence and -35 sequence

b.

recognition of the -10 sequence only

c.

recognition of the -35 sequence only

d.

none of the above

Discuss
Answer: (a).recognition of the -10 sequence and -35 sequence
Q58.
The transcriptional regulatory proteins in eukaryotes which induce transcription usually have

a.

at least two distinct domains of protein structure, a DNA binding domain and an activation domain

b.

at least two distinct domains of protein structure and a DNA binding domain

c.

at least one distinct domains of protein structure, a DNA binding domain and an activation domain

d.

none of these

Discuss
Answer: (a).at least two distinct domains of protein structure, a DNA binding domain and an activation domain
Q59.
In prokaryotes, transcription is

a.

terminated by the stop codon

b.

terminated by a protein called rho

c.

terminated by a poly A sequence

d.

terminated by a start codon

Discuss
Answer: (b).terminated by a protein called rho
Q60.
In eukaryotes, there are three different RNA polymerases. The RNA polymerase responsible for transcription of mRNA is

a.

RNA polymerase I

b.

RNA polymerase II

c.

RNA polymerase III

d.

none of these

Discuss
Answer: (b).RNA polymerase II
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