Recombinant DNA Technology MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Recombinant DNA Technology, a fundamental topic in the field of Biochemistry. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Recombinant DNA Technology MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Recombinant DNA Technology mcq questions that explore various aspects of Recombinant DNA Technology problems. Each MCQ is crafted to challenge your understanding of Recombinant DNA Technology principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biochemistry tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Recombinant DNA Technology MCQs are your pathway to success in mastering this essential Biochemistry topic.

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Recombinant DNA Technology MCQs | Page 4 of 21

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Q31.
The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ .

a.

would be of no concern if it occurred.

b.

has occurred

c.

can never occur

d.

seems unlikely

Discuss
Answer: (d).seems unlikely
Q32.
Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because

a.

in both cases molecules migrate to the anode

b.

both techniques rely on a constant charge to mass ratio

c.

both techniques utilize the sieving properties of gels

d.

all of the above

Discuss
Answer: (d).all of the above
Q33.
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

a.

copies of the gene with an altered sequence so that a restriction site is inserted

b.

mutagenized copies of a gene

c.

short sequences that will hybridize only to a specific base sequence

d.

PCR-amplified variable numbers of tandem repeats (VNTRs)

Discuss
Answer: (c).short sequences that will hybridize only to a specific base sequence
Q34.
The sequence of one strand of DNA is: 5' ATTGCCA 3', what is the sequence of the other strand?

a.

5' TAACGGT 3'

b.

5' TGGCAAT 3'

c.

5' ATTGCCA 3'

d.

5' UAAGCCU3'

Discuss
Answer: (b).5' TGGCAAT 3'
Q35.
In prokaryotes, AUG encodes

a.

methionine

b.

N-formyl methionine

c.

a stop codon

d.

alanine

Discuss
Answer: (b).N-formyl methionine
Q36.
How many amino acids will be encoded by 5' GAU GGU UGA UGU 3' sequence?

a.

One

b.

Two

c.

Three

d.

Four

Discuss
Answer: (b).Two
Q37.
In protein synthesis in prokaryotes

a.

the initiating amino acid is N- formyl methionine

b.

the initiating amino acid is methionine

c.

the initiating amino acid is phenyl alanine

d.

none of the above

Discuss
Answer: (a).the initiating amino acid is N- formyl methionine
Q38.
AUG codes for methionine act as a

a.

initiation code

b.

elongation code

c.

termination code

d.

propagation code

Discuss
Answer: (a).initiation code
Q39.
Which of the following techniques was carried out by Nirenberg and Matthaei in 1961 to determine the first codon?

a.

In vitro synthesis of a polypeptide using UUUUU

b.

Labeled peptide binding to a ribosome

c.

Mixed co-polymer mRNA synthesis

d.

none of the above

Discuss
Answer: (a).In vitro synthesis of a polypeptide using UUUUU
Q40.
Bacterial protein called catabolic activator protein (CAP) is an example of

a.

negative control of gene expression

b.

positive control of gene expression

c.

second type of positive control of gene expression

d.

none of the above

Discuss
Answer: (c).second type of positive control of gene expression
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