Recombinant DNA Technology MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Recombinant DNA Technology, a fundamental topic in the field of Biochemistry. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Recombinant DNA Technology MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Recombinant DNA Technology mcq questions that explore various aspects of Recombinant DNA Technology problems. Each MCQ is crafted to challenge your understanding of Recombinant DNA Technology principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biochemistry tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Recombinant DNA Technology MCQs are your pathway to success in mastering this essential Biochemistry topic.

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Recombinant DNA Technology MCQs | Page 4 of 21

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Q31.
The regions of a gene located at -10 and -35, with respect to the start of transcription, are termed

a.

start codons.

b.

oriC regions.

c.

Shine Dalgarno sequences.

d.

promoters.

Discuss
Answer: (d).promoters.
Q32.
Which of the following conditions would cause the release of the lac repressor protein from the lac operator site on DNA?

a.

Presence of glucose in the growth media

b.

Presence of lactose in the growth media

c.

Presence of IPTG (isopropyl thiogalactoside) in the growth media

d.

Both (b) and (c)

Discuss
Answer: (d).Both (b) and (c)
Q33.
The complex of RNA polymerase, DNA template and new RNA transcript is called

a.

transcription bubble

b.

replication bubble

c.

a translation bubble

d.

none of these

Discuss
Answer: (a).transcription bubble
Q34.
RNA polymerase in prokaryotes has a removable

a.

alpha subunit

b.

beta subunit

c.

both (a) and (b)

d.

sigma subunit

Discuss
Answer: (d).sigma subunit
Q35.
Promoters for tRNAs are located

a.

upstream from the start codon

b.

downstream from the start codon

c.

both (a) and (b)

d.

none of these

Discuss
Answer: (b).downstream from the start codon
Q36.
The binding of lac repressor to DNA could be considered to be analogous to

a.

competitive inhibition of an enzyme

b.

mixed-type inhibition of an enzyme

c.

uncompetitive inhibition of an enzyme

d.

allosteric effects in enzyme regulation

Discuss
Answer: (a).competitive inhibition of an enzyme
Q37.
Rho-dependent termination of transcription in E. coli

a.

requires ATP

b.

requires about 50 nucleotides of uncomplexed mRNA

c.

both (a) and (b)

d.

removes mRNA and holoenzyme from the DNA

Discuss
Answer: (c).both (a) and (b)
Q38.
Enhancers are regions that

a.

bind RNA polymerase

b.

are adjacent to the TATA box

c.

are CAT box binding proteins

d.

modulate transcription

Discuss
Answer: (d).modulate transcription
Q39.
Transcription by E. coli polymerase occurs in

a.

four phases known as initiation, propagation, elongation and termination

b.

three phases known as initiation, elongation and termination

c.

two phases known as initiation and termination

d.

none of the above

Discuss
Answer: (b).three phases known as initiation, elongation and termination
Q40.
Which of the following is necessary for transcription to occur?

a.

DNA molecule

b.

RNA polymerase

c.

DNA polymerase

d.

Both DNA & RNA polymerase

Discuss
Answer: (d).Both DNA & RNA polymerase
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