Animal Cell Culture and Regulation MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Animal Cell Culture and Regulation, a fundamental topic in the field of Biotechnology. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Animal Cell Culture and Regulation MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Animal Cell Culture and Regulation mcq questions that explore various aspects of Animal Cell Culture and Regulation problems. Each MCQ is crafted to challenge your understanding of Animal Cell Culture and Regulation principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biotechnology tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Animal Cell Culture and Regulation MCQs are your pathway to success in mastering this essential Biotechnology topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Animal Cell Culture and Regulation. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Animal Cell Culture and Regulation knowledge to the test? Let's get started with our carefully curated MCQs!

Animal Cell Culture and Regulation MCQs | Page 4 of 14

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Q31.
Disaggregating of cells can be achieved by

a.

physical disruption

b.

enzymatic digestion

c.

treating with chelating agents

d.

all of the above

Discuss
Answer: (d).all of the above
Q32.
Accumulation of lactate leads to

a.

increase in pH

b.

no change in pH

c.

reduction in the pH of culture hence loss of cell viability

d.

no loss of cell viability

Discuss
Answer: (c).reduction in the pH of culture hence loss of cell viability
Q33.
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

a.

incomplete glutamine oxidation

b.

increase in specific lactate production from glucose

c.

both (a) and (b)

d.

accumulation of ammonia

Discuss
Answer: (c).both (a) and (b)
Q34.
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

a.

6 amino acids

b.

8 amino acids

c.

10 amino acids

d.

12 amino acids

Discuss
Answer: (d).12 amino acids
Q35.
Excess CO2 suppress cell growth and productivity by

a.

inhibiting respiration

b.

altering intracellular pH by diffusing across cell membrane

c.

both (a) and (b)

d.

altering pH of the medium

Discuss
Answer: (c).both (a) and (b)
Q36.
Which of the following is incorrect?

a.

Established cell lines (ECL) have short doubling time

b.

ECL are invariably aneuploid

c.

ECL grow in higher density

d.

ECL do not show much evidence of spatial orientation

Discuss
Answer: (c).ECL grow in higher density
Q37.
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

a.

They act as growth promoters

b.

They act as growth inhibitors

c.

Have no effect on cells

d.

Lactate helps in the growth while ammonium inhibits the growth

Discuss
Answer: (d).Lactate helps in the growth while ammonium inhibits the growth
Q38.
What is the concentration of CO2 required for culturing animal cells?

a.

2-5%

b.

1-10%

c.

10-15%

d.

15-20%

Discuss
Answer: (b).1-10%
Q39.
Which of the following is correct?

a.

ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)

b.

ECL and PCL can be established in suspension cultures

c.

PCL can be established in suspension cultures

d.

none of the above

Discuss
Answer: (a).ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)
Q40.
Which of the followings are the metabolic products of glucose and glutamine?

a.

CO2 and NH3

b.

CO2 and lactate

c.

Lactate and ammonium

d.

Lactate only

Discuss
Answer: (c).Lactate and ammonium
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