| 1. | Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast? |
| a. | It is carried out with the help of micromanipulator |
| b. | The recipient cells are immobilized on artificial support or artificially bound to substarate |
| c. | It employs needle with diameter greater than cell diameter |
| d. | all of the above |
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Answer: (c).It employs needle with diameter greater than cell diameter
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| 2. | The right segment of octopine T-DNA (TR) is necessary for |
| a. | enzymes for agropine biosynthesis |
| b. | tumour formation |
| c. | conjugative transfer |
| d. | all of these |
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Answer: (a).enzymes for agropine biosynthesis
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| 3. | Opine synthesis is the property |
| a. | conferred to plant cells when it transformed by Agrobacterium tumefaciens |
| b. | determined by the bacteria Agrobacterium tumefaciens |
| c. | both (a) and (b) |
| d. | of normal plant cells |
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Answer: (c).both (a) and (b)
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| 4. | Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain |
| a. | one set of sequence necessary for gene transfer |
| b. | two sets of sequence necessary for gene transfer |
| c. | three sets of sequence necessary for gene transfer |
| d. | four sets of sequence necessary for gene transfer |
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Answer: (b).two sets of sequence necessary for gene transfer
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| 5. | The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as |
| a. | cos site |
| b. | flanking sequences |
| c. | border sequences |
| d. | transfer sequences |
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Answer: (c).border sequences
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