E-PolyLearning

31. Advantage of microprojectile method over microinjection method for gene transfer in plants include
a. intact cells are used
b. method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall
c. gene can be transferred to many cells simultaneously
d. all of the above
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Answer: (d).all of the above

32. On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of
a. 12 bp
b. 20 bp
c. 25 bp
d. 30 bp
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Answer: (c).25 bp

33. Agrobacterium tumefaciens is a
a. gram (+) bacteria
b. gram (-) bacteria
c. a fungi
d. a yeast
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Answer: (b).gram (-) bacteria

34. Virulence trait of Agrobacterium tumefaciens is borne on
a. chromosomal DNA
b. tumour inducing plasmid DNA
c. both chromosomal and plasmid DNA
d. cryptic plasmid DNA
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Answer: (b).tumour inducing plasmid DNA

35. The size of the virulent plasmid of Agrobacterium tumefaciens is
a. 40-80 kb
b. 80-120 kb
c. 140-235 kb
d. >235 kb
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Answer: (c).140-235 kb