Gene Transfer in Plants MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Gene Transfer in Plants, a fundamental topic in the field of Biotechnology. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Gene Transfer in Plants MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Gene Transfer in Plants mcq questions that explore various aspects of Gene Transfer in Plants problems. Each MCQ is crafted to challenge your understanding of Gene Transfer in Plants principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biotechnology tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Gene Transfer in Plants MCQs are your pathway to success in mastering this essential Biotechnology topic.

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Gene Transfer in Plants MCQs | Page 1 of 15

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Q1.
Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?

a.

It is carried out with the help of micromanipulator

b.

The recipient cells are immobilized on artificial support or artificially bound to substarate

c.

It employs needle with diameter greater than cell diameter

d.

all of the above

Discuss
Answer: (c).It employs needle with diameter greater than cell diameter
Q2.
The right segment of octopine T-DNA (TR) is necessary for

a.

enzymes for agropine biosynthesis

b.

tumour formation

c.

conjugative transfer

d.

all of these

Discuss
Answer: (a).enzymes for agropine biosynthesis
Q3.
Opine synthesis is the property

a.

conferred to plant cells when it transformed by Agrobacterium tumefaciens

b.

determined by the bacteria Agrobacterium tumefaciens

c.

both (a) and (b)

d.

of normal plant cells

Discuss
Answer: (c).both (a) and (b)
Q4.
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

a.

one set of sequence necessary for gene transfer

b.

two sets of sequence necessary for gene transfer

c.

three sets of sequence necessary for gene transfer

d.

four sets of sequence necessary for gene transfer

Discuss
Answer: (b).two sets of sequence necessary for gene transfer
Q5.
The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as

a.

cos site

b.

flanking sequences

c.

border sequences

d.

transfer sequences

Discuss
Answer: (c).border sequences
Q6.
Which of the following are used as selection marker for the cells transformed with Agrobacterium?

a.

Neomycin phosphotransferase

b.

Streptomycin phosphotransferase

c.

Hygromycin phosphotransferase

d.

any of the above

Discuss
Answer: (d).any of the above
Q7.
Vir genes required for the T-DNA transfer and processing are located

a.

on the T-DNA

b.

outside the T-DNA region

c.

on the plant genome

d.

none of these

Discuss
Answer: (b).outside the T-DNA region
Q8.
Plant transformation vectors based on Agrobacterium can generally be divided into

a.

two vectors

b.

four vectors

c.

six vectors

d.

eight vectors

Discuss
Answer: (a).two vectors
Q9.
Opines that are present in crown gall tumour include

a.

octopine

b.

nopaline

c.

agropine

d.

all of these

Discuss
Answer: (d).all of these
Q10.
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

a.

pRK 2013

b.

pRN 3

c.

either (a) or (b)

d.

none of these

Discuss
Answer: (c).either (a) or (b)
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