E-PolyLearning

6. Which of the following are used as selection marker for the cells transformed with Agrobacterium?
a. Neomycin phosphotransferase
b. Streptomycin phosphotransferase
c. Hygromycin phosphotransferase
d. any of the above
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Answer: (d).any of the above

7. Vir genes required for the T-DNA transfer and processing are located
a. on the T-DNA
b. outside the T-DNA region
c. on the plant genome
d. none of these
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Answer: (b).outside the T-DNA region

8. Plant transformation vectors based on Agrobacterium can generally be divided into
a. two vectors
b. four vectors
c. six vectors
d. eight vectors
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Answer: (a).two vectors

9. Opines that are present in crown gall tumour include
a. octopine
b. nopaline
c. agropine
d. all of these
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Answer: (d).all of these

10. Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?
a. pRK 2013
b. pRN 3
c. either (a) or (b)
d. none of these
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Answer: (c).either (a) or (b)

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