E-PolyLearning

11. Which of the following is true about T DNA?
a. Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
b. Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
c. Integration of T DNA occurs at two specific sites in the plant nuclear DNA
d. Integration of T DNA occurs at one site that may be random in the plant nuclear DNA
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Answer: (a).Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA

12. Which of the following is not true about the direct repeats flanking T-DNA?
a. They are conserved between nopaline and octopine Ti-plasmids
b. These repeats are transferred intact to the plant genome
c. These are important in integration mechanism
d. all of the above
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Answer: (b).These repeats are transferred intact to the plant genome

13. The left segment of octopine T-DNA (TL) is necessary for
a. enzymes for agropine biosynthesis
b. tumour formation
c. conjugative transfer
d. all of these
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Answer: (b).tumour formation

14. Microprojectile method of gene transfer in plants involves delivery of DNA
a. with the help of micromanipulator
b. with the help of bolistics
c. with the help of needles
d. any of the above
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Answer: (b).with the help of bolistics

15. Which of the following genes are constitutively expressed and control the plant induced activation of other vir genes?
a. vir A and vir G
b. vir C and vir D
c. vir B and vir E
d. vir A and vir B
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Answer: (a).vir A and vir G

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