Q. Which of the following statements are true about the roles of RNA polymerase subunits?

i. α subunit is encoded by “rpo A” gene, required for core protein assembly.
ii. β subunit is encoded by “rpo C” gene is the catalytic center of RNA polymerase.
iii. ω subunit is encoded by “rpo Z” gene and helps in proper association of all the subunits.
iv. σ factor is encoded by “rpo D” gene and contributes in promoter recognition.

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Q. The α subunits of polymerase has a function of ____________

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Q. The β subunit of polymerase has a function of ____________

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Q. Presence of the heparin promotes transcription.

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Q. The β’ subunit of polymerase has a function of ________________

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Q. The RNA polymerase core enzyme converts into a holoenzyme on the addition of the _________ subunit.

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Q. Four types of σ factors are known of them which one is heat stable?

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Q. What is the function of the ω subunit of RNA polymerase?

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Q. Which of the following is not part of the RNA polymerase core enzyme?

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Q. The bacterial system has _____________ RNA polymerases.

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Q. What is the consensus sequence of the Pribnow box?

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Q. The Pribnow box is present on the coding strand of the DNA template.

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Q. The –35 sequence is highly conserved and has a consensus sequence of ___________

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Q. The +1 site of conserved promoter sequence is generally a pyrimidine.

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Q. –10, – 35 and +1 sites are the consensus promoter sites of sigma factor ______________

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Q. The RNA polymerase core enzyme has a high specificity for promoter sites and can perfectly work alone.

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Q. The complex formed by the polymerase and the promoter DNA is known as the _____________

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Q. Negative supercoiling enhances the rate of transcription.

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Q. The –10 promoter region is rich in ______________ bases.

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Q. Without using primers for RNA synthesis the polymerase produces small stretches of RNA of _____________ nucleotides.

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