Q. If a parent yeast is of mating type a, what will be the mating type of its bud?

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Q. Under which condition will the yeast express a-alpha mating type?

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Q. RAP1 binds to ____________

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Q. Which of these SIR proteins are having HDAC activity?

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Q. You transfer the DAM gene to the telomere of yeast. What will be your expected result?

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Q. What is the range of telomere suppression?

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Q. In which of the following chromosome compaction is absent?

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Q. Histones are __________

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Q. Which of the following will increase transcription?

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Q. Which histone(s) are acted upon by HAT?

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Q. In a substrate X catabolic gene promoter you replace the normal histones by your experimental histones – ones with arginine side chains in place of histidine. What will be the effect on the concentration of substrate X against the control?

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Q. You take a crude cell lysate and treat it with DNase. Now wash it and add protease. Use specific restriction endonuclease to get your desired fragment and run it on a gel. You see a single band. Which of the following will be true about the fragment?

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Q. Which of the following is not a common mode of histone modification in eukaryotes?

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Q. Which of the following association acts as transcriptional activator?

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Q. In an experiment you perform the following- you attach a flag epitope to HDAC, you over express a mutated Mad1-pro gene within the experimental cell, you immunoprecipitate the protein complex using flag epitope and run that on a non-denaturing SDS PAGE and western blot. Now you add antibodies for HDAC, MAD1 and SIN3A stripping after each addition. What will you observe?

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Q. In presence of Thyroid hormone the receptor TR binds to ____________

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Q. If the events in post transcriptional modification are listed as-

i) Polyadenylation ii) capping iii) splicing

What would be the correct sequence?

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Q. Which RNA transcript would be capped by the capping enzymes in vitro mixture of RNA?

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Q. Which nucleotide is present in the 5’ cap?

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Q. Which is the 1st enzyme in capping?

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