Q. These are steps taken in carrying out the PCR reaction:

i) Attaching of primers by cooling
ii) Denaturation of strands
iii) DNA synthesis
iv) Heating

Which is the correct order?

(Mentioned from starting to ending the reaction)

View solution

Q. Which of the following is not a condition for PCR?

View solution

Q. Primers and polymerases are added again during the reaction because they get consumed as the reaction proceeds.

View solution

Q. All the molecules generated during PCR will not be full length. Some will also be of intermediate length. Which of the statements is correct?

View solution

Q. Which of the following activity is not present in Taq polymerase?

View solution

Q. Choose the correct statement.

View solution

Q. What is the half life cycle for Taq polymerase?

View solution

Q. Taq polymerase incorporates which residue at 3’ end?

View solution

Q. Polymerases are also available from other Thermus species. Which of the following is correct?

View solution

Q. Polymerases are available with proof reading activity. Which of the following are the characteristics of these types of polymerases?

View solution

Q. Thermococcus litoralis grows at a temperature upto 98 degrees.

View solution

Q. Primers are generally ____________

View solution

Q. Which end of the primer should be matched properly in order to carry out the amplification?

View solution

Q. Which of the following nucleotides should be there at 3’ end?

View solution

Q. Melting temperature is given by ____________

View solution

Q. Both the primers, the start primer and the end primer should have a nearly same melting temperature.

View solution

Q. Why internal secondary structures are not preferred for primers?

View solution

Q. Which of the following is favoured for primer design?

View solution

Q. What will happen if the amino acid sequence is used directly for primer designing?

View solution

Q. In the case of uncertainty, if more than one nucleotide is included at a position it is called ____________

View solution