Q. At times, the gene which is cloned is not well known for the protein encoded by it. To access the function, the endogenous gene for the mutant strain is inactivated. This technique is called as ___________

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Q. Approaches for creating mutations can be divided into how many types?

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Q. In the case of deletion of a restriction site, it should be cleaved with the same restriction enzyme.

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Q. If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be?

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Q. If a functional gene is disrupted while disrupting a restriction site _______ is created.

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Q. Additional restriction sites can be introduced near the existing restriction site by mutagenesis.

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Q. Restriction site can also be introduced by oligonucleotide directed mutagenesis of a region that is _______ and ______ to the original restriction site.

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Q. Small deletions at a restriction site can be generated by cutting and degrading the ________ with an exonuclease.

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Q. When a series of deleted regions are replaced by some other DNA fragment of equal length, then it is known as:

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Q. Bisulphite ions are used to deaminate ________ residues in _______ DNA.

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Q. For mutagenesis without PCR, which of the following can be used as a template?

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Q. An oligonucleotide is synthesized which contains the mutation and the rest is _______ to the template DNA.

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Q. If the template is double stranded, they need to be separated before annealing of oligonucleotide.

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Q. Which of the following statement is incorrect for the synthesis of the second strand?

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Q. Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced?

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Q. How many sites can be mutated at a time?

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Q. Several different mutations can be induced at one site.

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Q. If mixed oligonucleotides are used, it is regarded as ______________

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Q. It is easier to subclone a restriction fragment if it belongs to?

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Q. A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.

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