DNA and Genomic Sequencing MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on DNA and Genomic Sequencing, a fundamental topic in the field of Bioinformatics. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our DNA and Genomic Sequencing MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of DNA and Genomic Sequencing mcq questions that explore various aspects of DNA and Genomic Sequencing problems. Each MCQ is crafted to challenge your understanding of DNA and Genomic Sequencing principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Bioinformatics tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our DNA and Genomic Sequencing MCQs are your pathway to success in mastering this essential Bioinformatics topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of DNA and Genomic Sequencing. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your DNA and Genomic Sequencing knowledge to the test? Let's get started with our carefully curated MCQs!

DNA and Genomic Sequencing MCQs | Page 1 of 17

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Q1.
Which of the following is untrue about DNA sequencing methods?

a.

Purified fragments of DNA cut from plasmid/phage clones or amplified by polymerase chain reaction (PCR)

b.

Clones of DNA fragments are denatured to single strands, and one of the strands is hybridized to an oligonucleotide primer

c.

Taq polymerase is quite heat sensitive

d.

New strands of DNA are synthesized from the end of the primer

Discuss
Answer: (c).Taq polymerase is quite heat sensitive
Q2.
Using ddATP, the resulting synthesis creates a set of nested DNA fragments, each one ending at one of the as in the sequence through the substitution of a fluorescent-labeled ddATP.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q3.
The combined mixture of all labeled DNA fragments is electrophoresed to _____ the fragments by______ and the ladder of fragments is scanned for the presence of each of the four labels.

a.

separate, size

b.

separate, pH

c.

assimilate, pH

d.

assimilate, size

Discuss
Answer: (a).separate, size
Q4.
The sequence can also be verified by making an oligonucleotide primer complementary to the distal part of the readable sequence and using it to obtain the sequence of the complementary strand on the original DNA template.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q5.
When the process is fully automated, a number of priming sites may be used to obtain sequencing results that give optimal separation of bands in each region of the sequence.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q6.
To sequence larger molecules, individual chromosomes are purified and broken into _____ or larger random fragments, which are cloned into vectors designed for large molecules.

a.

100-Mb

b.

100-kb

c.

5000-kb

d.

600-kb

Discuss
Answer: (b).100-kb
Q7.
Many levels of clone redundancy may be required to build a consensus map because individual clones can have _______

a.

rearrangements

b.

deletions

c.

two separate fragments

d.

vectors

Discuss
Answer: (d).vectors
Q8.
Once the correct map has been obtained, unique overlapping clones are chosen for sequencing.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q9.
An alternative method is to sequence all the subclones, produce a computer database of the sequences, and then have the computer assemble the sequences from the overlaps that are found.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q10.
Which of the following is untrue about Shotgun Sequencing?

a.

When DNA fragments derived from different chromosomal regions have repeats of the same sequence, they will appear to overlap

b.

When DNA fragments derived from different chromosomal regions have repeats of the same sequence, they will appear to scrutinize

c.

In a new whole shotgun approach, Celera Genomics is sequencing both ends of DNA fragments of short (2 kb), medium (10 kb), and long (BAC or >100 kb) lengths

d.

A large number of reads are then assembled by computer

Discuss
Answer: (b).When DNA fragments derived from different chromosomal regions have repeats of the same sequence, they will appear to scrutinize
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