Recombinant DNA MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Recombinant DNA, a fundamental topic in the field of Biotechnology. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Recombinant DNA MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Recombinant DNA mcq questions that explore various aspects of Recombinant DNA problems. Each MCQ is crafted to challenge your understanding of Recombinant DNA principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Biotechnology tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Recombinant DNA MCQs are your pathway to success in mastering this essential Biotechnology topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Recombinant DNA. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Recombinant DNA knowledge to the test? Let's get started with our carefully curated MCQs!

Recombinant DNA MCQs | Page 2 of 4

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Q11.
Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

a.

DNA ligase

b.

helicases

c.

restriction endonucleases

d.

DNA gyrase

Discuss
Answer: (c).restriction endonucleases
Q12.
Which of the following vector can maintain the largest fragment of foreign DNA?

a.

YAC

b.

Cosmid

c.

Plasmid

d.

Phage

Discuss
Answer: (a).YAC
Q13.
Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

a.

photoreactivation

b.

gel electrophoresis

c.

autoradiography

d.

blotting

Discuss
Answer: (b).gel electrophoresis
Q14.
For gene probes to be useful they must

a.

be large enough to contain gene-specific sequences

b.

be labeled in some manner to allow detection

c.

both (a) and (b)

d.

none of the above

Discuss
Answer: (c).both (a) and (b)
Q15.
A genomic library is

a.

a database where the sequence of an organism's genome is stored

b.

a collection of many clones possessing different DNA fragments from the same organisms bound to vectors

c.

a book that describes how to isolate DNA from a particular organism

d.

a place where the information of the genetic organization of organisms are kept.

Discuss
Answer: (b).a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
Q16.
Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

a.

DNA polymerase

b.

Restriction endonuclease

c.

DNA ligase

d.

DNA helicase

Discuss
Answer: (c).DNA ligase
Q17.
Bacterial cells protect their own DNA from degradation by restriction endonucleases by

a.

methylating the DNA at the sites that the enzyme recognizes

b.

deleting all recognition sites from the genome

c.

not producing any restriction endonucleases

d.

having anti restriction endonucleases

Discuss
Answer: (a).methylating the DNA at the sites that the enzyme recognizes
Q18.
Which type of restriction endonuclease cuts the DNA within the recognition site?

a.

Type I

b.

Type II

c.

Type III

d.

All of the these

Discuss
Answer: (b).Type II
Q19.
A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

a.

southern blotting

b.

northern blotting

c.

polymerase chain reaction

d.

DNA replication

Discuss
Answer: (c).polymerase chain reaction
Q20.
The Southern blotting technique depends on

a.

similarities between the sequences of probe DNA and experimental DNA

b.

similarities between the sequences of probe RNA and experimental RNA

c.

similarities between the sequences of probe protein and experimental protein

d.

the molecular mass of proteins

Discuss
Answer: (a).similarities between the sequences of probe DNA and experimental DNA
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