E-PolyLearning

11. Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are
a. DNA ligase
b. helicases
c. restriction endonucleases
d. DNA gyrase
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Answer: (c).restriction endonucleases

12. Which of the following vector can maintain the largest fragment of foreign DNA?
a. YAC
b. Cosmid
c. Plasmid
d. Phage
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Answer: (a).YAC

13. Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as
a. photoreactivation
b. gel electrophoresis
c. autoradiography
d. blotting
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Answer: (b).gel electrophoresis

14. For gene probes to be useful they must
a. be large enough to contain gene-specific sequences
b. be labeled in some manner to allow detection
c. both (a) and (b)
d. none of the above
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Answer: (c).both (a) and (b)

15. A genomic library is
a. a database where the sequence of an organism's genome is stored
b. a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
c. a book that describes how to isolate DNA from a particular organism
d. a place where the information of the genetic organization of organisms are kept.
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Answer: (b).a collection of many clones possessing different DNA fragments from the same organisms bound to vectors

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