| 11. | Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are |
| a. | DNA ligase |
| b. | helicases |
| c. | restriction endonucleases |
| d. | DNA gyrase |
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Answer: (c).restriction endonucleases
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| 12. | Which of the following vector can maintain the largest fragment of foreign DNA? |
| a. | YAC |
| b. | Cosmid |
| c. | Plasmid |
| d. | Phage |
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Answer: (a).YAC
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| 13. | Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as |
| a. | photoreactivation |
| b. | gel electrophoresis |
| c. | autoradiography |
| d. | blotting |
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Answer: (b).gel electrophoresis
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| 14. | For gene probes to be useful they must |
| a. | be large enough to contain gene-specific sequences |
| b. | be labeled in some manner to allow detection |
| c. | both (a) and (b) |
| d. | none of the above |
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Answer: (c).both (a) and (b)
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| 15. | A genomic library is |
| a. | a database where the sequence of an organism's genome is stored |
| b. | a collection of many clones possessing different DNA fragments from the same organisms bound to vectors |
| c. | a book that describes how to isolate DNA from a particular organism |
| d. | a place where the information of the genetic organization of organisms are kept. |
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Answer: (b).a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
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