| 16. | Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid? |
| a. | DNA polymerase |
| b. | Restriction endonuclease |
| c. | DNA ligase |
| d. | DNA helicase |
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Answer: (c).DNA ligase
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| 17. | Bacterial cells protect their own DNA from degradation by restriction endonucleases by |
| a. | methylating the DNA at the sites that the enzyme recognizes |
| b. | deleting all recognition sites from the genome |
| c. | not producing any restriction endonucleases |
| d. | having anti restriction endonucleases |
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Answer: (a).methylating the DNA at the sites that the enzyme recognizes
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| 18. | Which type of restriction endonuclease cuts the DNA within the recognition site? |
| a. | Type I |
| b. | Type II |
| c. | Type III |
| d. | All of the these |
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Answer: (b).Type II
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| 19. | A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as |
| a. | southern blotting |
| b. | northern blotting |
| c. | polymerase chain reaction |
| d. | DNA replication |
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Answer: (c).polymerase chain reaction
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| 20. | The Southern blotting technique depends on |
| a. | similarities between the sequences of probe DNA and experimental DNA |
| b. | similarities between the sequences of probe RNA and experimental RNA |
| c. | similarities between the sequences of probe protein and experimental protein |
| d. | the molecular mass of proteins |
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Answer: (a).similarities between the sequences of probe DNA and experimental DNA
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