16. | Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid? |
a. | DNA polymerase |
b. | Restriction endonuclease |
c. | DNA ligase |
d. | DNA helicase |
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Answer: (c).DNA ligase
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17. | Bacterial cells protect their own DNA from degradation by restriction endonucleases by |
a. | methylating the DNA at the sites that the enzyme recognizes |
b. | deleting all recognition sites from the genome |
c. | not producing any restriction endonucleases |
d. | having anti restriction endonucleases |
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Answer: (a).methylating the DNA at the sites that the enzyme recognizes
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18. | Which type of restriction endonuclease cuts the DNA within the recognition site? |
a. | Type I |
b. | Type II |
c. | Type III |
d. | All of the these |
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Answer: (b).Type II
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19. | A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as |
a. | southern blotting |
b. | northern blotting |
c. | polymerase chain reaction |
d. | DNA replication |
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Answer: (c).polymerase chain reaction
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20. | The Southern blotting technique depends on |
a. | similarities between the sequences of probe DNA and experimental DNA |
b. | similarities between the sequences of probe RNA and experimental RNA |
c. | similarities between the sequences of probe protein and experimental protein |
d. | the molecular mass of proteins |
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Answer: (a).similarities between the sequences of probe DNA and experimental DNA
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