E-PolyLearning

16. Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?
a. DNA polymerase
b. Restriction endonuclease
c. DNA ligase
d. DNA helicase
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Answer: (c).DNA ligase

17. Bacterial cells protect their own DNA from degradation by restriction endonucleases by
a. methylating the DNA at the sites that the enzyme recognizes
b. deleting all recognition sites from the genome
c. not producing any restriction endonucleases
d. having anti restriction endonucleases
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Answer: (a).methylating the DNA at the sites that the enzyme recognizes

18. Which type of restriction endonuclease cuts the DNA within the recognition site?
a. Type I
b. Type II
c. Type III
d. All of the these
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Answer: (b).Type II

19. A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as
a. southern blotting
b. northern blotting
c. polymerase chain reaction
d. DNA replication
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Answer: (c).polymerase chain reaction

20. The Southern blotting technique depends on
a. similarities between the sequences of probe DNA and experimental DNA
b. similarities between the sequences of probe RNA and experimental RNA
c. similarities between the sequences of probe protein and experimental protein
d. the molecular mass of proteins
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Answer: (a).similarities between the sequences of probe DNA and experimental DNA

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