Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 19 of 24

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Q181.
If blunt ended ligations are to be carried out. Which of the following enzymes can be used?

a.

E. coli DNA ligase

b.

T4 DNA ligase

c.

Both of these enzymes act equally in carrying out blunt ended ligations

d.

None of them is able to carry out blunt ended ligations

Discuss
Answer: (b).T4 DNA ligase
Q182.
E. coli DNA ligase doesn’t require a cofactor.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q183.
Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?

a.

AMP is added to the 5’ phosphate of one of the DNA molecule

b.

It leads to the liberation of pyrophosphate from NAD and nicotinamide mononucleotide from ATP

c.

The AMP is further displaced by an electrophilic attack

d.

The AMP is further displaced by nucleophilic attack by 3’ hydroxyl of the same DNA molecule

Discuss
Answer: (a).AMP is added to the 5’ phosphate of one of the DNA molecule
Q184.
Topoisomerase is also an enzyme which is used for carrying out ligation. The correct statement for topoisomerase is?

a.

They act only on double stranded molecules

b.

They alter the degree of supercoiling of DNA molecules

c.

They are less effective than conventional DNA ligase

d.

There are three types of topoisomerases

Discuss
Answer: (b).They alter the degree of supercoiling of DNA molecules
Q185.
Mobile genetic elements can be transferred from one DNA portion to another. The enzyme carrying out this is ____________

a.

Ligase

b.

Transciptase

c.

Transposase

d.

Endonuclease

Discuss
Answer: (c).Transposase
Q186.
Phage based recombination systems are used for ____________

a.

cleaving the molecules at specific sites

b.

adding the molecules at specific sites

c.

breakage and rejoining the molecules at specific sites

d.

breakage at random sites

Discuss
Answer: (c).breakage and rejoining the molecules at specific sites
Q187.
Bacteriophage lambda is having a phage recombination system. Following are the characteristics of this system ____________

a.

It is used for inserting phage genome into the bacterium

b.

It is used for inserting bacterial genome into the phage

c.

The specific site in bacteria is attB and that in phage is attP

d.

The specific sites in both of them are called as attP

Discuss
Answer: (a).It is used for inserting phage genome into the bacterium
Q188.
Ligation enzymes are used for ligating newly synthesized okazaki fragments. What holds true for okazaki fragments?

a.

Okazaki fragments are short fragments of DNA formed on the leading strand

b.

Okazaki fragments are large fragments of DNA formed on the lagging strand

c.

Okazaki fragments are short fragments of DNA formed on the lagging strand

d.

Okazaki fragments are large fragments of DNA formed on the leading strand

Discuss
Answer: (c).Okazaki fragments are short fragments of DNA formed on the lagging strand
Q189.
Introduction of DNA molecules into the recipient organism is termed as ____________

a.

transformation

b.

translation

c.

transduction

d.

transcription

Discuss
Answer: (a).transformation
Q190.
Many bacterial species are having a natural ability to take up the exogenous DNA material. Which of the statement is not correct in regard to it?

a.

This ability is termed as competence

b.

It is not limited to particular growth phases

c.

The bacteria may develop new biochemical abilities under special conditions such as nutrient deprivation

d.

Induction of specific set of bacterial proteins may take place

Discuss
Answer: (b).It is not limited to particular growth phases
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