Basics of Genetic Engineering MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Basics of Genetic Engineering, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Basics of Genetic Engineering MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Basics of Genetic Engineering mcq questions that explore various aspects of Basics of Genetic Engineering problems. Each MCQ is crafted to challenge your understanding of Basics of Genetic Engineering principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Basics of Genetic Engineering MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Basics of Genetic Engineering MCQs | Page 16 of 24

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Q151.
The E. coli DNA polymerase enzyme gives different domains with different activities on cleaving with protease subtilisin. Which of the following statements is correct with respect to the fragments generated?

a.

The smaller fragment is having C terminal and the larger fragment is having N terminal

b.

The smaller fragment is named as Klenow fragment and the intact molecule is called as Kornberg fragment

c.

The smaller fragment is having 5’-3’ exonuclease activity whereas the larger fragment is having 5’-3’ polymerase and 3’-5’ exonuclease activity

d.

Both the fragments are having 5’-3’ polymerase and 3’-5’ exonuclease activity

Discuss
Answer: (c).The smaller fragment is having 5’-3’ exonuclease activity whereas the larger fragment is having 5’-3’ polymerase and 3’-5’ exonuclease activity
Q152.
Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________

a.

polishing and end filling respectively

b.

end filling and polishing respectively

c.

polishing in both the cases

d.

end filling in both the cases

Discuss
Answer: (a).polishing and end filling respectively
Q153.
Thermostable DNA polymerases are very important in PCR. How are they obtained?

a.

They are obtained by heating the bacteria manually over high temperatures

b.

They are isolated from extremely stable thermophilic bacteria which are often found growing in oceanic vents

c.

They are found everywhere in nature

d.

They are obtained by genetically modifying the E. coli bacteria with thermal stability property

Discuss
Answer: (b).They are isolated from extremely stable thermophilic bacteria which are often found growing in oceanic vents
Q154.
Which of the following enzyme is said as reverse transcriptase?

a.

DNA dependent DNA polymerase

b.

RNA dependent RNA polymerase

c.

RNA dependent DNA polymerase

d.

DNA dependent RNA polymerase

Discuss
Answer: (c).RNA dependent DNA polymerase
Q155.
Why reverse transcriptase enzymes are having comparatively high error rates than other polymerases?

a.

Because they are not having 3’-5’ proofreading exonucleolytic activity

b.

Because they are not having 5’-3’ proofreading exonucleolytic activity

c.

Because they are having slow rates of exonucleolytic activity

d.

It is difficult to synthesize DNA from RNA

Discuss
Answer: (a).Because they are not having 3’-5’ proofreading exonucleolytic activity
Q156.
Which polymerase can be used in conjunction with appropriate phage promoters in order to have high levels of specific transcription?

a.

RNA dependent DNA polymerase

b.

DNA dependent RNA polymerase

c.

RNA dependent RNA polymerase

d.

DNA dependent DNA polymerase

Discuss
Answer: (b).DNA dependent RNA polymerase
Q157.
Template independent polymerases are the enzymes which add nucleotide bases without a template. Which of the following statements is correct with respect to these?

a.

They only add a single nucleotide

b.

They only add a string of nucleotides and not a single nucleotide

c.

Terminal transferase adds a series of nucleotides at the 3’ end

d.

Taq polymerase adds a single nucleotide at the 5’ end of the PCR product

Discuss
Answer: (c).Terminal transferase adds a series of nucleotides at the 3’ end
Q158.
Which of the following statements is correct with respect to exonuclease?

a.

They only act on single stranded DNA molecules

b.

They only act on double stranded DNA molecules

c.

They remove a single nucleotide base at a time

d.

They remove nucleotide bases from the middle of a polynucleotide chain

Discuss
Answer: (c).They remove a single nucleotide base at a time
Q159.
How many approaches are there which can be used for exonucleolytic activity in double stranded DNA molecules?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q160.
What is the mode of action of exonuclease III?

a.

Exonuclease III acts on single stranded DNA in 3’-5’ direction

b.

Exonuclease III acts on double stranded DNA in 5’-3’ direction

c.

Exonuclease III acts on single stranded DNA in 5’-3’ direction

d.

Exonuclease III acts on double stranded DNA in 3’-5’ direction

Discuss
Answer: (d).Exonuclease III acts on double stranded DNA in 3’-5’ direction
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