Polymerase Chain Reaction MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Polymerase Chain Reaction, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Polymerase Chain Reaction MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Polymerase Chain Reaction mcq questions that explore various aspects of Polymerase Chain Reaction problems. Each MCQ is crafted to challenge your understanding of Polymerase Chain Reaction principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Polymerase Chain Reaction MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Polymerase Chain Reaction. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Polymerase Chain Reaction knowledge to the test? Let's get started with our carefully curated MCQs!

Polymerase Chain Reaction MCQs | Page 1 of 8

Q1.
The process of amplification of specific DNA sequences by an enzymatic process is termed as ____________
Discuss
Answer: (a).amplification
Discuss
Answer: (b).Primers are the short sequences which are complementary to the nucleotides at the end of the sequence which is to be amplified
Discuss
Answer: (c).deoxynucleoside triphosphate (dNTPs)
Discuss
Answer: (b).It is heat stable
Q5.
These are steps taken in carrying out the PCR reaction:

i) Attaching of primers by cooling
ii) Denaturation of strands
iii) DNA synthesis
iv) Heating

Which is the correct order?

(Mentioned from starting to ending the reaction)
Discuss
Answer: (d).iv)-ii)-i)-iii)
Discuss
Answer: (c).Renaturation for 5 minutes at 60 degrees
Q7.
Primers and polymerases are added again during the reaction because they get consumed as the reaction proceeds.
Discuss
Answer: (b).False
Discuss
Answer: (b).In the next cycle, each intermediate molecule will generate one intermediate molecule and one target molecule
Discuss
Answer: (c).3’-5’ exonuclease
Discuss
Answer: (b).Processivity is defined in this case as a synthesis of DNA by polymerase
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