Polymerase Chain Reaction MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Polymerase Chain Reaction, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Polymerase Chain Reaction MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Polymerase Chain Reaction mcq questions that explore various aspects of Polymerase Chain Reaction problems. Each MCQ is crafted to challenge your understanding of Polymerase Chain Reaction principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Polymerase Chain Reaction MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Polymerase Chain Reaction. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Polymerase Chain Reaction knowledge to the test? Let's get started with our carefully curated MCQs!

Polymerase Chain Reaction MCQs | Page 3 of 8

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Q21.
Why internal secondary structures are not preferred for primers?

a.

Internal structures are very bulky and thus elongation is not preferred

b.

Because of it, primer may fold back on itself and won’t be available for template

c.

Internal secondary structures require more amount of template

d.

If internal structures are present, no proof reading would be observed

Discuss
Answer: (b).Because of it, primer may fold back on itself and won’t be available for template
Q22.
Which of the following is favoured for primer design?

a.

The melting temperature should be different for both the primers

b.

Primers should be long in length

c.

Primers should not be complementary to each other

d.

Matching should be of whole primer to the template

Discuss
Answer: (c).Primers should not be complementary to each other
Q23.
What will happen if the amino acid sequence is used directly for primer designing?

a.

There would be certainty because the genetic code is unique for each amino acid

b.

There would be uncertainty as the genetic code is degenerate and none of the amino acid is having a unique code

c.

There would be uncertainty as the genetic code is degenerate but some of the amino acids such as methionine are having a unique codon

d.

The amount of uncertainty or certainty is a matter of chance

Discuss
Answer: (c).There would be uncertainty as the genetic code is degenerate but some of the amino acids such as methionine are having a unique codon
Q24.
In the case of uncertainty, if more than one nucleotide is included at a position it is called ____________

a.

mixed site

b.

polynucleotide site

c.

unique site

d.

degenerate site

Discuss
Answer: (a).mixed site
Q25.
What is the property of inosine?

a.

Having narrow range of pairing capabilities

b.

Having a broad range of pairing capabilities

c.

The pairing capability is the same as the normal nucleotides

d.

It is abbreviated as A

Discuss
Answer: (b).Having a broad range of pairing capabilities
Q26.
Which of the following is useful in applications of PCR?

a.

It is manual

b.

Only one sample’s analysis can be carried out at a time

c.

It is having a high speed

d.

The amount of DNA required initially is high

Discuss
Answer: (c).It is having a high speed
Q27.
What is the correct statement with respect to ddNTPs?

a.

They are dideoxynucleotide triphosphates

b.

They are used in the termination of DNA sequencing

c.

They are used for initiating DNA sequencing

d.

They are used in the case if the starting amounts are large

Discuss
Answer: (b).They are used in the termination of DNA sequencing
Q28.
Cycle sequencing is the DNA sequencing where very less amounts of template are utilised for carrying out the sequencing.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q29.
Sickle cell anaemia is a genetic disorder. Which of the following doesn’t holds true for it?

a.

It can be analysed by PCR

b.

It destroys a restriction site

c.

The mutation is in alpha globulin gene

d.

The conventional approach took weeks for the whole analyses to be carried out

Discuss
Answer: (c).The mutation is in alpha globulin gene
Q30.
PCR products can be analysed in many ways. Which of the following is not possible?

a.

Use of restriction enzymes

b.

Determining whether a particular oliginucleotide probe hybridizes to a PCR product

c.

Electrophoresis

d.

Direct sequencing can’t be carried out

Discuss
Answer: (d).Direct sequencing can’t be carried out
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