Polymerase Chain Reaction MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Polymerase Chain Reaction, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Polymerase Chain Reaction MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Polymerase Chain Reaction mcq questions that explore various aspects of Polymerase Chain Reaction problems. Each MCQ is crafted to challenge your understanding of Polymerase Chain Reaction principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Polymerase Chain Reaction MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Polymerase Chain Reaction. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Polymerase Chain Reaction knowledge to the test? Let's get started with our carefully curated MCQs!

Polymerase Chain Reaction MCQs | Page 7 of 8

Discover more Topics under Genetic Engineering

Q61.
Reverse transciptase PCR is also carried out at times. Which of the statement is true?

a.

Amplification of RNA samples is not required for knowing the abundance of mRNA

b.

Both the start and the end primers are used

c.

Only a single cDNA strand is synthesized before the PCR

d.

The primer used is always specific

Discuss
Answer: (d).The primer used is always specific
Q62.
Which type of PCR allows the use of permeabilized tissue?

a.

Inverse PCR

b.

In-situ PCR

c.

Quantitative PCR

d.

Hot-start PCR

Discuss
Answer: (b).In-situ PCR
Q63.
How many approaches are there for measuring the quantity of PCR products?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q64.
Which of the statement hold true for Quantitative PCR?

a.

A fluorescent dye is used which binds on single stranded DNA molecules

b.

SYBR green is an example such type of dye

c.

The quantity of DNA is simply measured by measuring the amount of fluorescence

d.

This approach is useful if the products are non-specific in nature

Discuss
Answer: (c).The quantity of DNA is simply measured by measuring the amount of fluorescence
Q65.
In another method of quantitative PCR, reporter-quencher set up is used. Which of the statement holds true for this methodology?

a.

It allows detection of all double stranded molecules

b.

The reporter and quencher are the molecules present on the same probe

c.

The quencher is having a fluorescent group

d.

Fluorescence is observed only when both the groups are present in proximity to each other

Discuss
Answer: (b).The reporter and quencher are the molecules present on the same probe
Q66.
By using two oligonucleotides, we can measure the amount of quantity of DNA by measuring the amount of fluorescence. The first probe absorbs light and the second emits it at a particular wavelength.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q67.
Choose the correct statement with regard to quantitative PCR?

a.

End-point PCR is favourable over real time PCR

b.

In real time PCR, quantification is done as the reaction is going on

c.

If the product measurement is done after the completion then the measurement is done by target sequence and no other factor affects it

d.

If the primers are available in limited amount, then the product obtained is proportional to the target sequence

Discuss
Answer: (b).In real time PCR, quantification is done as the reaction is going on
Q68.
Mutation is never deliberately induced in PCR products.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q69.
If amplification of one of the strand is favoured, the modification of PCR is known as __________

a.

single-strand PCR

b.

partial PCR

c.

asymmetric PCR

d.

anchored PCR

Discuss
Answer: (c).asymmetric PCR
Q70.
Which of the statement is incorrect for anchored PCR?

a.

Anchored PCR is the modification in which only one piece of the sequence is known and thus one primer

b.

The known sequence is attached to the required region of amplification and then further used as a second priming site

c.

Fragment the sample DNA and ligate it to known sequence

d.

Tails are added enzymatically to the region of known sequence

Discuss
Answer: (d).Tails are added enzymatically to the region of known sequence
Page 7 of 8