Polymerase Chain Reaction MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Polymerase Chain Reaction, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Polymerase Chain Reaction MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Polymerase Chain Reaction mcq questions that explore various aspects of Polymerase Chain Reaction problems. Each MCQ is crafted to challenge your understanding of Polymerase Chain Reaction principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Polymerase Chain Reaction MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Polymerase Chain Reaction. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

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Polymerase Chain Reaction MCQs | Page 1 of 8

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Q1.
The process of amplification of specific DNA sequences by an enzymatic process is termed as ____________

a.

amplification

b.

polymerase chain reaction (PCR)

c.

translation

d.

microarrays

Discuss
Answer: (a).amplification
Q2.
What are primers?

a.

Primers are the short sequences at the end of the nucleotide sequences which are used for amplification

b.

Primers are the short sequences which are complementary to the nucleotides at the end of the sequence which is to be amplified

c.

Primers are the short sequences present anywhere in the nucleotide sequence to be amplified

d.

Primers are the short sequences which are complementary to the nucleotides anywhere in the sequence to be amplified

Discuss
Answer: (b).Primers are the short sequences which are complementary to the nucleotides at the end of the sequence which is to be amplified
Q3.
A reaction mixture for PCR consists of ____________

a.

heat unstable polymerase

b.

primers in a limited amount

c.

deoxynucleoside triphosphate (dNTPs)

d.

a region complementary to the sequence to be amplified

Discuss
Answer: (c).deoxynucleoside triphosphate (dNTPs)
Q4.
Which of the following is a characteristic of Taq polymerase?

a.

It is an RNA polymerase

b.

It is heat stable

c.

It is obtained from thermophilic bacterium and can be grown in the laboratory below a temperature of 75 degrees

d.

It is used in cellular synthesis processes and the optimum temperature is at least 90 degrees

Discuss
Answer: (b).It is heat stable
Q5.
These are steps taken in carrying out the PCR reaction:

i) Attaching of primers by cooling
ii) Denaturation of strands
iii) DNA synthesis
iv) Heating

Which is the correct order?

(Mentioned from starting to ending the reaction)

a.

i)-ii)-iii)-iv)

b.

ii)-i)-iii)-iv)

c.

iv)-iii)-ii)-i)

d.

iv)-ii)-i)-iii)

Discuss
Answer: (d).iv)-ii)-i)-iii)
Q6.
Which of the following is not a condition for PCR?

a.

Initial melting carried out for 5 minutes at 94 degrees

b.

Initial melting followed by 30 cycles each consisting of melting for 1 minute at 94 degrees

c.

Renaturation for 5 minutes at 60 degrees

d.

DNA synthesis at 72 degrees for 1.5 minutes

Discuss
Answer: (c).Renaturation for 5 minutes at 60 degrees
Q7.
Primers and polymerases are added again during the reaction because they get consumed as the reaction proceeds.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q8.
All the molecules generated during PCR will not be full length. Some will also be of intermediate length. Which of the statements is correct?

a.

After first cycle, majority of the molecules will be full length and only some will be of intermediate length

b.

In the next cycle, each intermediate molecule will generate one intermediate molecule and one target molecule

c.

The number of full length molecules increase as number of cycles proceed

d.

The number of intermediate molecules increase geometrically and the number of target molecules increase arithmetically

Discuss
Answer: (b).In the next cycle, each intermediate molecule will generate one intermediate molecule and one target molecule
Q9.
Which of the following activity is not present in Taq polymerase?

a.

5’-3’ polymerase

b.

5’-3’ exonuclease

c.

3’-5’ exonuclease

d.

Both 5’-3’ polymerase and 5’-3’ exonuclease

Discuss
Answer: (c).3’-5’ exonuclease
Q10.
Choose the correct statement.

a.

Taq polymerase is having high processivity

b.

Processivity is defined in this case as a synthesis of DNA by polymerase

c.

It requires a 5’ end for the elongation to take place

d.

The maximum size of the molecules which can be synthesized is 10kbp

Discuss
Answer: (b).Processivity is defined in this case as a synthesis of DNA by polymerase
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