Simple Cloning MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Simple Cloning, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Simple Cloning MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Simple Cloning mcq questions that explore various aspects of Simple Cloning problems. Each MCQ is crafted to challenge your understanding of Simple Cloning principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Simple Cloning MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Simple Cloning. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Simple Cloning knowledge to the test? Let's get started with our carefully curated MCQs!

Simple Cloning MCQs | Page 1 of 8

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Q1.
Transformation efficiency is defined as __________

a.

ratio of transformed colonies by microgram of sample DNA that is to be inserted

b.

ratio of transformed colonies by amount of sample DNA that is to be inserted

c.

ratio of transformed colonies by microgram of plasmid DNA

d.

ratio of transformed colonies by amount of plasmid DNA

Discuss
Answer: (d).ratio of transformed colonies by amount of plasmid DNA
Q2.
After carrying out the cloning experiment, the cells are plated on agar. Along with agar, it also contains antibiotic resistant genes, X-gal and an inducer of lacZ gene. Which of the following would grow?

a.

Cells that have taken up plasmid DNA

b.

Cells that have taken up genomic DNA

c.

Cells having no insert

d.

Cells having no insert

Discuss
Answer: (a).Cells that have taken up plasmid DNA
Q3.
Choose the correct statement for adaptors.

a.

They are blunt ended at both the ends

b.

They are single stranded at both the ends

c.

They may be single stranded at one end and other end may be blunt

d.

They don’t have extra restriction sites within their sequence

Discuss
Answer: (c).They may be single stranded at one end and other end may be blunt
Q4.
If linkers are combined with other features such as a selectable marker, it is called as __________

a.

cassette

b.

modified linker

c.

adaptors

d.

induced linker

Discuss
Answer: (a).cassette
Q5.
Plasmids are used for carrying out the cloning procedure. Which of the statement is true for plasmids?

a.

Bacterial plasmids are linear in nature

b.

They are single stranded

c.

Insertion of DNA into plasmid allows it to be propagated in host cells and they are known as vectors because of their this property

d.

They are not capable of replication in bacteria

Discuss
Answer: (c).Insertion of DNA into plasmid allows it to be propagated in host cells and they are known as vectors because of their this property
Q6.
Which of the following characteristic is not present in a plasmid on a general basis?

a.

Multiple cloning site (MCS)

b.

Origin of replication (ori)

c.

Antibiotic resistance gene

d.

Beta galactose genes

Discuss
Answer: (d).Beta galactose genes
Q7.
Bla is a gene, which is incorrect for it?

a.

It is an antibiotic resistance genes

b.

Antibiotic acts by blocking the cross-linking of the bacterial cell wall and thus leading to lysing of cells

c.

It encodes beta lactamase enzyme

d.

The beta lactam ring is activated

Discuss
Answer: (d).The beta lactam ring is activated
Q8.
Which of the characteristics is present in lacZ gene?

a.

It encodes for beta galactosidase enzyme

b.

Beta galactosidase enzyme is responsible for cleaving monosaccharides into the constituent elements

c.

It doesn’t cleaves a substrate called as X-gal

d.

But if X-gal is cleaved, it liberates pink coloured dye

Discuss
Answer: (a).It encodes for beta galactosidase enzyme
Q9.
Which of the following orientation is not propagated to later stages?

a.

Circular plasmids having genomic DNA

b.

Intramolecular ligation of plasmids

c.

Linear molecules

d.

Head to head ligation of two DNA plasmids

Discuss
Answer: (c).Linear molecules
Q10.
Molecules having new combination of sequences that were not present before are called as __________

a.

intermolecular ligants

b.

recombinants

c.

couple

d.

intramolecular ligants

Discuss
Answer: (b).recombinants
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