Simple Cloning MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Simple Cloning, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Simple Cloning MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Simple Cloning mcq questions that explore various aspects of Simple Cloning problems. Each MCQ is crafted to challenge your understanding of Simple Cloning principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Simple Cloning MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Simple Cloning. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Simple Cloning knowledge to the test? Let's get started with our carefully curated MCQs!

Simple Cloning MCQs | Page 4 of 8

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Q31.
The cell in which the recombinant molecules are propagated is termed as __________

a.

host

b.

vector

c.

plasmid

d.

carrier

Discuss
Answer: (a).host
Q32.
Some mutations are present which assist the uptake of DNA. Which of the statement is incorrect for it?

a.

deoR is such a mutation which assists the uptake of DNA

b.

It is a transcriptional regulator

c.

It is having DNA binding activity

d.

It controls the expression of a set of genes involved ribonucleoside catabolism

Discuss
Answer: (d).It controls the expression of a set of genes involved ribonucleoside catabolism
Q33.
Endogenous DNA-degrading system is not essential in determining transformation efficiency.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q34.
Which of the following is true for K strain for E. coli?

a.

It contains restriction-modification system which is encoded by hsDMRS locus

b.

hsDR encodes for endonuclease which is responsible for cleaving the DNA sequence containing –AACGCNNTGC-

c.

Cleavage is carried out when the second A is non methylated

d.

hsDR mutation can be used for cleavage of incoming unprotected DNA

Discuss
Answer: (a).It contains restriction-modification system which is encoded by hsDMRS locus
Q35.
Methylation dependent restriction system (MDRS) are included in which class of restriction enzymes?

a.

Type I

b.

Type II

c.

Type III

d.

They are the product of mcrA, mcrB or mrr loci

Discuss
Answer: (d).They are the product of mcrA, mcrB or mrr loci
Q36.
Choose the incorrect statement with respect to dam and dcm genes?

a.

Their enzymes are responsible for methylation of DNA

b.

Dam protein methylates cytosine in –GATC-

c.

Dcm methylates cytosine in-CCAGG- and –CCTGG- sequences

d.

The extent of methylation also has effects on the efficiency with which the other restriction enzymes cleave the DNA in vitro

Discuss
Answer: (b).Dam protein methylates cytosine in –GATC-
Q37.
How many recombination systems are present in E. coli?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (c).3
Q38.
Disablement is also done in plasmids. Choose the correct statement.

a.

The strains carrying out the recombinant plasmids should not escape outside

b.

Preferred strains are having mutations which allow their growth in wild

c.

Mutations confer prototrophy

d.

If the recombinants escape outside, there is no such harm

Discuss
Answer: (a).The strains carrying out the recombinant plasmids should not escape outside
Q39.
endA mutation is often induced in host molecules. Choose the correct statement.

a.

It activates the gene for DNA-specific endonuclease

b.

It enhances the yield of plasmid DNA preparations only

c.

It enhances the quality of plasmid DNA preparations only

d.

It enhances both the quality and yield of plasmid DNA preparations

Discuss
Answer: (d).It enhances both the quality and yield of plasmid DNA preparations
Q40.
lacZΞ”M15 represents __________

a.

fully present lacZ gene

b.

it is not required in alpha complementation

c.

it represents the lacZ gene with M15 mutation

d.

it is partially deleted lacZ gene which is needed for alpha complementation

Discuss
Answer: (d).it is partially deleted lacZ gene which is needed for alpha complementation
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