Simple Cloning MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Simple Cloning, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Simple Cloning MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Simple Cloning mcq questions that explore various aspects of Simple Cloning problems. Each MCQ is crafted to challenge your understanding of Simple Cloning principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Simple Cloning MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Simple Cloning. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Simple Cloning knowledge to the test? Let's get started with our carefully curated MCQs!

Simple Cloning MCQs | Page 5 of 8

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Q41.
The correct statement with respect to lacIq is __________

a.

It is a mutant of lac repressor gene and decreases the level of repressor

b.

It doesn’t control the lacZ gene

c.

It is important for the vectors to replicate in a low copy number

d.

It doesn’t titrate out the repressor produced by wild type Lac-I gene

Discuss
Answer: (a).It is a mutant of lac repressor gene and decreases the level of repressor
Q42.
There are various methods to distinguish whether a colony contains a recombinant or not. One such method is __________

a.

blue white screening

b.

checking whether replication is taking place or not

c.

checking the number of copies

d.

looking for the multiple cloning site

Discuss
Answer: (a).blue white screening
Q43.
At times it is observed that non-recombinant plasmids are more than that of recombinant plasmids. Choose the correct statement in regard to it.

a.

It makes easier to get the recombinant plasmids from the mixture

b.

The ratio of insert DNA to vector DNA is reduced to get recombinant plasmids

c.

Alkaline phosphatase is used to get recombinant plasmids

d.

Alkaline phosphatase method is less reliable in comparison to the ratio method

Discuss
Answer: (c).Alkaline phosphatase is used to get recombinant plasmids
Q44.
Alkaline Phosphatase is used at times and the vector is treated with it. Choose the incorrect statement.

a.

It removes 5’ terminal phosphate group from nucleic acids

b.

The 5’ phosphate group is required for the ligation to take place

c.

Two phosphate bonds should be formed for the complete ligation to take place

d.

The ligation between vector and insert won’t take place

Discuss
Answer: (d).The ligation between vector and insert won’t take place
Q45.
What is the correct time for carrying out the alkaline phosphatase treatment?

a.

After the cutting of vector has been done

b.

Before the insert DNA and the vector are mixed

c.

After the cutting of vector has been done but before the insert DNA and vector are mixed

d.

After the mixing of insert DNA and vector

Discuss
Answer: (c).After the cutting of vector has been done but before the insert DNA and vector are mixed
Q46.
ccdB gene is used at times. Choose the correct statement with respect to this gene.

a.

It is a control of cell birth gene

b.

It is activated in complex vectors to increase the fraction of recombinants

c.

If intramolecular ligation takes place this protein is released

d.

It is often regarded as control of cell death and birth gene

Discuss
Answer: (b).It is activated in complex vectors to increase the fraction of recombinants
Q47.
When inserting a DNA fragment, it is possible to have two orientations. If the orientation is controlled, this cloning is referred to as __________

a.

forced cloning

b.

orientation cloning

c.

correct cloning

d.

restricted cloning

Discuss
Answer: (a).forced cloning
Q48.
What happens if insert DNA is cut with two different restriction enzymes at the ends?

a.

It is difficult to insert the fragment

b.

The insert can be ligated in any orientation

c.

The insert can be ligated in one orientation only

d.

The amount of product increases

Discuss
Answer: (c).The insert can be ligated in one orientation only
Q49.
What is the disadvantage of amplification of using PCR over natural cloning?

a.

In PCR, there is no proof reading activity

b.

In PCR, small fragments can’t be amplified

c.

There is an A incorporated in PCR products, which makes cloning difficult

d.

PCR takes more time as compared to natural cloning

Discuss
Answer: (a).In PCR, there is no proof reading activity
Q50.
TA cloning is a method used for cloning of PCR products. Which of the statement is correct with respect to it?

a.

It is based on the fact that a T residue is incorporated at the end of the PCR product

b.

‘A’ residue is incorporated into the end of the vector

c.

It gives a low yield

d.

It is preferred over blunt end ligation

Discuss
Answer: (d).It is preferred over blunt end ligation
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