Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 1 of 12

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Q1.
At times streptavidin is used in place of cellulose. Choose the correct statement for this alternative.

a.

oligo-dT streptavidin conjugate can be extracted by magnetic beads

b.

The magnetic beads are attached with activin

c.

Recovery is not based on the magnetic properties of the beads

d.

The interaction between polyA tail and the oligo-dT is reduced

Discuss
Answer: (a).oligo-dT streptavidin conjugate can be extracted by magnetic beads
Q2.
Choose the correct with respect to RNA molecules.

a.

They are less labile than DNA molecules

b.

The 2’ hydroxyl group of ribose group decreases the activity

c.

There is no less of activity while boiling

d.

Baking of glassware, treatment with UV can be used for protection against degradation

Discuss
Answer: (d).Baking of glassware, treatment with UV can be used for protection against degradation
Q3.
What is the basis of RNaseH method?

a.

It is based on RNA synthesis by DNA strand

b.

It is based on complementary DNA synthesis by RNA strand through reverse transcriptase

c.

It is based on complementary DNA synthesis by RNA strand through RNaseH enzyme

d.

It is based on getting double strand RNA from a single strand

Discuss
Answer: (b).It is based on complementary DNA synthesis by RNA strand through reverse transcriptase
Q4.
The first step for RNaseH method is to anneal a chemically synthesized oligo-dT primer to the 3’ polyA tail of RNA.

a.

True

b.

False

c.

May be True or False

d.

Can't Say

Discuss
Answer: (a).True
Q5.
For the synthesis of the second DNA strand, incorporated oligo-dT is of no use. Why?

a.

It is so because it is unstable

b.

It is so because it is at the 3’ end of the template molecule

c.

It is so because it would be unable to initiate replication

d.

It is so because it detaches itself after first strand synthesis

Discuss
Answer: (c).It is so because it would be unable to initiate replication
Q6.
Choose the incorrect statement for the homopolymer tailing of cDNA strands.

a.

Choose the incorrect statement for the homopolymer tailing of cDNA strands.

b.

Vector is also treated with terminal transferase and dGTPs

c.

The vector and cDNA can now anneal with the help of DNA ligase

d.

If gaps are created they can be repaired by physiological processes

Discuss
Answer: (c).The vector and cDNA can now anneal with the help of DNA ligase
Q7.
Choose the correct statement if the RNA is non polydenylated.

a.

A collection of chemically synthesized oligonucleotides is used as primers

b.

They are usually tetramer

c.

Unequal quantities of A, G, T and C are used

d.

The primers attach at only specific sequences for first strand synthesis

Discuss
Answer: (a).A collection of chemically synthesized oligonucleotides is used as primers
Q8.
In case if molecules smaller than the fragments required for making a full genomic library are used for making a collection. This collection is called as ___________

a.

library

b.

shelf

c.

small library

d.

mini library

Discuss
Answer: (b).shelf
Q9.
Choose the correct statement for construction of a library subsection.

a.

The size of a particular restriction fragment on which the gene is located is not known

b.

The size of the restriction fragment can be known by carrying out southern blotting

c.

Another digest of the genomic DNA is carried out by a different enzyme

d.

DNA fragments of different size are recovered after carrying out gel electrophoresis

Discuss
Answer: (b).The size of the restriction fragment can be known by carrying out southern blotting
Q10.
Any cDNA library would represent a fraction of RNA species of an organism.

a.

True

b.

False

c.

May be True or False

d.

Can't Say

Discuss
Answer: (a).True
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