Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 4 of 12

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Q31.
Choose the correct statement for modification of homopolymer tailing method.

a.

It includes modification of primers

b.

Primers are varied by simply altering their size by randomly adding or removing bases

c.

The 5’ end of the first cDNA strand is tailed with C residues

d.

A single stranded oilgonucleotide is then used for second strand synthesis

Discuss
Answer: (a).It includes modification of primers
Q32.
By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q33.
Sometimes the required mRNA is present in less number. So the process of increasing the representation of rare mRNAs is called as ____________

a.

amplification

b.

normalization

c.

selection

d.

narrowing

Discuss
Answer: (b).normalization
Q34.
Choose the correct statement in order to enrich rare species.

a.

The most basic principle relies on the kinetics of hybridization

b.

A collection of single stranded molecules is allowed to reanneal under given conditions

c.

The strands which are less abundant are able to find their complement easily

d.

The left over molecules are more abundant in nature

Discuss
Answer: (a).The most basic principle relies on the kinetics of hybridization
Q35.
Which statement holds true for hydroxyapatite?

a.

It binds to single stranded molecules more tightly than double stranded molecules

b.

It binds to linear molecules more tightly than circular molecules

c.

It binds to circular molecules more tightly than linear molecules

d.

It binds to double stranded molecules more tightly than single stranded molecules

Discuss
Answer: (d).It binds to double stranded molecules more tightly than single stranded molecules
Q36.
At times streptavidin is used in place of cellulose. Choose the correct statement for this alternative.

a.

oligo-dT streptavidin conjugate can be extracted by magnetic beads

b.

The magnetic beads are attached with activin

c.

Recovery is not based on the magnetic properties of the beads

d.

The interaction between polyA tail and the oligo-dT is reduced

Discuss
Answer: (a).oligo-dT streptavidin conjugate can be extracted by magnetic beads
Q37.
Choose the correct with respect to RNA molecules.

a.

They are less labile than DNA molecules

b.

The 2’ hydroxyl group of ribose group decreases the activity

c.

There is no less of activity while boiling

d.

Baking of glassware, treatment with UV can be used for protection against degradation

Discuss
Answer: (d).Baking of glassware, treatment with UV can be used for protection against degradation
Q38.
What is the basis of RNaseH method?

a.

It is based on RNA synthesis by DNA strand

b.

It is based on complementary DNA synthesis by RNA strand through reverse transcriptase

c.

It is based on complementary DNA synthesis by RNA strand through RNaseH enzyme

d.

It is based on getting double strand RNA from a single strand

Discuss
Answer: (b).It is based on complementary DNA synthesis by RNA strand through reverse transcriptase
Q39.
The first step for RNaseH method is to anneal a chemically synthesized oligo-dT primer to the 3’ polyA tail of RNA.

a.

True

b.

False

c.

May be True or False

d.

Can't Say

Discuss
Answer: (a).True
Q40.
For the synthesis of the second DNA strand, incorporated oligo-dT is of no use. Why?

a.

It is so because it is unstable

b.

It is so because it is at the 3’ end of the template molecule

c.

It is so because it would be unable to initiate replication

d.

It is so because it detaches itself after first strand synthesis

Discuss
Answer: (c).It is so because it would be unable to initiate replication
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