Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 4 of 12

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Q31.
For cloning purposes, the intact chromosomes should be separated by ___________

a.

agarose gel electrophoresis

b.

fluorescence- activated sorter

c.

polyacrylamide gel electrophoresis

d.

chromatography

Discuss
Answer: (b).fluorescence- activated sorter
Q32.
The process of examining stained chromosomes in a light microscope and removing appropriate regions with a micro-manipulator is called as ___________

a.

microdissection

b.

chromosome sorting

c.

chromosome walking

d.

chromosome jumping

Discuss
Answer: (a).microdissection
Q33.
If the library to be screened and the host belongs to different species, then it is called as ___________

a.

homologous selection

b.

heterologous selection

c.

intraspecies selection

d.

mixed selection

Discuss
Answer: (b).heterologous selection
Q34.
The direct selection allows _______ number of recombinants to be screened and _______

a.

less, slowly

b.

less, quickly

c.

more, quickly

d.

more, slowly

Discuss
Answer: (c).more, quickly
Q35.
Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.

a.

DNA from collections is transcribed within in vitro and the transcription products are microinjected into oocytes

b.

The RNA is translated within oocytes

c.

Screening is carried out on the basis of DNA

d.

The screening is helpful for the proteins whose function can be easily screened such as transport of ions

Discuss
Answer: (c).Screening is carried out on the basis of DNA
Q36.
In the case of ligand binding by the expressed protein, the library can be screened by ______________

a.

In the case of ligand binding by the expressed protein, the library can be screened by ______________

b.

ligands if the sequence we are looking for encodes a protein specific to a ligand

c.

using a specific DNA sequence which can bind to the protein encoded by the sequence of interest

d.

all of the mentioned

Discuss
Answer: (d).all of the mentioned
Q37.
Choose the incorrect statement for cDNA libraries.

a.

They constitute of DNA copies produced from the RNA sequences and usually mRNA

b.

They represent expressed sequences

c.

Introns are not represented

d.

Comparison of cDNA sequences with genomic sequences leads to the determination of polyadenylation sites

Discuss
Answer: (c).Introns are not represented
Q38.
A times partial sequencing of cloned cDNAs is carried out. These cDNA are known as ___________

a.

expressed RNA sequences

b.

expressed sequence tags (ESTs)

c.

expressed cDNA sequences

d.

library

Discuss
Answer: (b).expressed sequence tags (ESTs)
Q39.
Polyadenylation of RNA species is an important criterion for the production of cDNA species. Which of the following holds true?

a.

Polyadenylation should be at 3’ end

b.

Eukaryotic mRNAs are mostly non-polyadenylated

c.

Bacterial mRNAs and organelle mRNAs are polyadenylated

d.

It is carried out by the addition of T residues after synthesis

Discuss
Answer: (a).Polyadenylation should be at 3’ end
Q40.
Choose the incorrect statement for oligo-dT cellulose.

a.

It is used for separation of polyadenylated mRNA from another mRNA

b.

oligo-dT are covalently attached to the solid support via OH bonds

c.

A solution containing RNA is passed through the column

d.

Poly A tail attaches to the oligo-dT by ionic bonds

Discuss
Answer: (d).Poly A tail attaches to the oligo-dT by ionic bonds
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