Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Genomic and Screening Libraries. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Genomic and Screening Libraries knowledge to the test? Let's get started with our carefully curated MCQs!

Genomic and Screening Libraries MCQs | Page 4 of 12

Discover more Topics under Genetic Engineering

Q31.
What is done after RNA fractionation is carried out?

a.

Each band is translated in vivo

b.

Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells

c.

Translation is carried out with a high background

d.

Amino acid is not radioactively labelled

Discuss
Answer: (b).Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells
Q32.
The polypeptides produced after addition of mRNA are analysed with antibodies. Choose the incorrect statement for this analysis.

a.

Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction

b.

Along with simple antigen-antibody complex, a substrate is added for easy precipitation

c.

Protein A-Sepharose is added

d.

Protein A-Sepharose binds to IgG antibodies

Discuss
Answer: (a).Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction
Q33.
Poly A tail from the column is eluted by using high salt concentration.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q34.
Choose the incorrect statement for second DNA strand synthesis.

a.

The RNA: DNA complex is treated with the enzyme RNaseH and DNA pol

b.

The enzyme RNaseH is responsible for nicking the RNA strand and leaving free 3’ hydroxyl ends

c.

These RNA fragments can be used as primers

d.

There is no portion of RNA left attached to the DNA strand

Discuss
Answer: (d).There is no portion of RNA left attached to the DNA strand
Q35.
What is the final product of the RNaseH method?

a.

blunt ended dsDNA

b.

staggered dsDNA at both ends

c.

staggered dsDNA at 3’ end

d.

staggered dsDNA at 5’ end

Discuss
Answer: (a).blunt ended dsDNA
Q36.
What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?

a.

There are no chances of the synthesis of the second DNA strand

b.

Chance complementarity would take place

c.

Hairpin structure would be formed

d.

Hairpin structure is formed is not the final structure

Discuss
Answer: (a).There are no chances of the synthesis of the second DNA strand
Q37.
The loop region is single stranded. It can be cleaved by using which enzyme?

a.

Exonuclease

b.

S1 nuclease

c.

RNaseH

d.

DNase

Discuss
Answer: (b).S1 nuclease
Q38.
Choose the correct statement with respect to the self priming method of cDNA synthesis.

a.

It is less preferred than RNaseH method

b.

A hairpin structure is formed with guarantee

c.

The sequence corresponding to the 5’ end is lost

d.

Reverse transcriptase is not used

Discuss
Answer: (c).The sequence corresponding to the 5’ end is lost
Q39.
Choose the incorrect statement for the method homopolymer tailing.

a.

The first step is the RNA: DNA hybrid synthesis

b.

Terminal transferase is used for the addition of nucleotides on 3’ end

c.

Terminal transferase adds only at DNA strands

d.

The DNA strand is now having known sequence at 3’ end

Discuss
Answer: (c).Terminal transferase adds only at DNA strands
Q40.
Choose the correct statement for RACE.

a.

It stands for Random Amplification of cDNA ends

b.

It is for cloning particular cDNA ends

c.

It is only of one type, which is 5’ RACE

d.

Sequence data is not available in any case

Discuss
Answer: (b).It is for cloning particular cDNA ends
Page 4 of 12