Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 4 of 12

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Q31.
Choose the correct statement with respect to the self priming method of cDNA synthesis.

a.

It is less preferred than RNaseH method

b.

A hairpin structure is formed with guarantee

c.

The sequence corresponding to the 5’ end is lost

d.

Reverse transcriptase is not used

Discuss
Answer: (c).The sequence corresponding to the 5’ end is lost
Q32.
Choose the incorrect statement for the method homopolymer tailing.

a.

The first step is the RNA: DNA hybrid synthesis

b.

Terminal transferase is used for the addition of nucleotides on 3’ end

c.

Terminal transferase adds only at DNA strands

d.

The DNA strand is now having known sequence at 3’ end

Discuss
Answer: (c).Terminal transferase adds only at DNA strands
Q33.
Choose the correct statement for RACE.

a.

It stands for Random Amplification of cDNA ends

b.

It is for cloning particular cDNA ends

c.

It is only of one type, which is 5’ RACE

d.

Sequence data is not available in any case

Discuss
Answer: (b).It is for cloning particular cDNA ends
Q34.
The first primer in the case of 3’ RACE is ___________

a.

internal sequence

b.

oligo-dT adaptor molecule

c.

oligo-dA adaptor molecule

d.

adaptor oligo-dT primer

Discuss
Answer: (b).oligo-dT adaptor molecule
Q35.
The first cDNA strand in 5’ RACE is tailed with oligo-dA tail.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q36.
What is the second primer in the case of 5’ RACE?

a.

Internal primer

b.

Oligo-dA sequence

c.

Adaptor-oligo-dT primer

d.

Oligo-dT adaptor molecule

Discuss
Answer: (c).Adaptor-oligo-dT primer
Q37.
RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

a.

Blunt ended ligation

b.

Addition of linkers

c.

Using appropriate restriction enzymes

d.

All the methods can be used equivalently

Discuss
Answer: (d).All the methods can be used equivalently
Q38.
Choose the correct statement for modification of homopolymer tailing method.

a.

It includes modification of primers

b.

Primers are varied by simply altering their size by randomly adding or removing bases

c.

The 5’ end of the first cDNA strand is tailed with C residues

d.

A single stranded oilgonucleotide is then used for second strand synthesis

Discuss
Answer: (a).It includes modification of primers
Q39.
In case if molecules smaller than the fragments required for making a full genomic library are used for making a collection. This collection is called as ___________

a.

library

b.

shelf

c.

small library

d.

mini library

Discuss
Answer: (b).shelf
Q40.
Choose the correct statement for construction of a library subsection.

a.

The size of a particular restriction fragment on which the gene is located is not known

b.

The size of the restriction fragment can be known by carrying out southern blotting

c.

Another digest of the genomic DNA is carried out by a different enzyme

d.

DNA fragments of different size are recovered after carrying out gel electrophoresis

Discuss
Answer: (b).The size of the restriction fragment can be known by carrying out southern blotting
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