Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 4 of 12

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Q31.
Choose the correct statement with respect to the self priming method of cDNA synthesis.

a.

It is less preferred than RNaseH method

b.

A hairpin structure is formed with guarantee

c.

The sequence corresponding to the 5’ end is lost

d.

Reverse transcriptase is not used

Discuss
Answer: (c).The sequence corresponding to the 5’ end is lost
Q32.
Choose the incorrect statement for the method homopolymer tailing.

a.

The first step is the RNA: DNA hybrid synthesis

b.

Terminal transferase is used for the addition of nucleotides on 3’ end

c.

Terminal transferase adds only at DNA strands

d.

The DNA strand is now having known sequence at 3’ end

Discuss
Answer: (c).Terminal transferase adds only at DNA strands
Q33.
Choose the correct statement for RACE.

a.

It stands for Random Amplification of cDNA ends

b.

It is for cloning particular cDNA ends

c.

It is only of one type, which is 5’ RACE

d.

Sequence data is not available in any case

Discuss
Answer: (b).It is for cloning particular cDNA ends
Q34.
In case if molecules smaller than the fragments required for making a full genomic library are used for making a collection. This collection is called as ___________

a.

library

b.

shelf

c.

small library

d.

mini library

Discuss
Answer: (b).shelf
Q35.
Choose the correct statement for construction of a library subsection.

a.

The size of a particular restriction fragment on which the gene is located is not known

b.

The size of the restriction fragment can be known by carrying out southern blotting

c.

Another digest of the genomic DNA is carried out by a different enzyme

d.

DNA fragments of different size are recovered after carrying out gel electrophoresis

Discuss
Answer: (b).The size of the restriction fragment can be known by carrying out southern blotting
Q36.
Any cDNA library would represent a fraction of RNA species of an organism.

a.

True

b.

False

c.

May be True or False

d.

Can't Say

Discuss
Answer: (a).True
Q37.
What do we mean by housekeeping genes?

a.

Housekeeping genes are those genes which are specific to an organism

b.

Housekeeping genes are those genes which are present in all the organisms

c.

Housekeeping genes are those genes which are meant for repair and maintenance in a species of organism

d.

Housekeeping genes are those genes which required for the replication process

Discuss
Answer: (b).Housekeeping genes are those genes which are present in all the organisms
Q38.
Choose the correct statement for RNA fractionation.

a.

The RNA is fractioned by size but before separating on oligo-dT cellulose

b.

A sucrose density gradient is used

c.

The RNA is applied to the top of a pre-poured gradient and during centrifugation smaller molecules move down the tube faster

d.

Different bands are formed according to the density in the sucrose density gradient

Discuss
Answer: (b).A sucrose density gradient is used
Q39.
What is done after RNA fractionation is carried out?

a.

Each band is translated in vivo

b.

Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells

c.

Translation is carried out with a high background

d.

Amino acid is not radioactively labelled

Discuss
Answer: (b).Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells
Q40.
The polypeptides produced after addition of mRNA are analysed with antibodies. Choose the incorrect statement for this analysis.

a.

Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction

b.

Along with simple antigen-antibody complex, a substrate is added for easy precipitation

c.

Protein A-Sepharose is added

d.

Protein A-Sepharose binds to IgG antibodies

Discuss
Answer: (a).Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction
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