Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 4 of 12

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Q31.
Polyadenylation of RNA species is an important criterion for the production of cDNA species. Which of the following holds true?

a.

Polyadenylation should be at 3’ end

b.

Eukaryotic mRNAs are mostly non-polyadenylated

c.

Bacterial mRNAs and organelle mRNAs are polyadenylated

d.

It is carried out by the addition of T residues after synthesis

Discuss
Answer: (a).Polyadenylation should be at 3’ end
Q32.
Choose the incorrect statement for oligo-dT cellulose.

a.

It is used for separation of polyadenylated mRNA from another mRNA

b.

oligo-dT are covalently attached to the solid support via OH bonds

c.

A solution containing RNA is passed through the column

d.

Poly A tail attaches to the oligo-dT by ionic bonds

Discuss
Answer: (d).Poly A tail attaches to the oligo-dT by ionic bonds
Q33.
Poly A tail from the column is eluted by using high salt concentration.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q34.
Choose the incorrect statement for second DNA strand synthesis.

a.

The RNA: DNA complex is treated with the enzyme RNaseH and DNA pol

b.

The enzyme RNaseH is responsible for nicking the RNA strand and leaving free 3’ hydroxyl ends

c.

These RNA fragments can be used as primers

d.

There is no portion of RNA left attached to the DNA strand

Discuss
Answer: (d).There is no portion of RNA left attached to the DNA strand
Q35.
What is the final product of the RNaseH method?

a.

blunt ended dsDNA

b.

staggered dsDNA at both ends

c.

staggered dsDNA at 3’ end

d.

staggered dsDNA at 5’ end

Discuss
Answer: (a).blunt ended dsDNA
Q36.
What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?

a.

There are no chances of the synthesis of the second DNA strand

b.

Chance complementarity would take place

c.

Hairpin structure would be formed

d.

Hairpin structure is formed is not the final structure

Discuss
Answer: (a).There are no chances of the synthesis of the second DNA strand
Q37.
What is done after the recovery of pellets has been carried out in order to know the amount of polypeptides?

a.

Denaturing and gel electrophoresis in SDS- Polyacryamide gel

b.

Gel electrophoresis in agarose gel

c.

Quantitative PCR

d.

Weighing pellets

Discuss
Answer: (a).Denaturing and gel electrophoresis in SDS- Polyacryamide gel
Q38.
Sometimes a gene which we want to clone is present on a particular chromosome. For this purpose, the chromosome should be in which phase?

a.

Prophase

b.

Telophase

c.

Metaphase

d.

Anaphase

Discuss
Answer: (c).Metaphase
Q39.
For cloning purposes, the intact chromosomes should be separated by ___________

a.

agarose gel electrophoresis

b.

fluorescence- activated sorter

c.

polyacrylamide gel electrophoresis

d.

chromatography

Discuss
Answer: (b).fluorescence- activated sorter
Q40.
The process of examining stained chromosomes in a light microscope and removing appropriate regions with a micro-manipulator is called as ___________

a.

microdissection

b.

chromosome sorting

c.

chromosome walking

d.

chromosome jumping

Discuss
Answer: (a).microdissection
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