Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 5 of 12

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Q41.
If the library to be screened and the host belongs to different species, then it is called as ___________

a.

homologous selection

b.

heterologous selection

c.

intraspecies selection

d.

mixed selection

Discuss
Answer: (b).heterologous selection
Q42.
The direct selection allows _______ number of recombinants to be screened and _______

a.

less, slowly

b.

less, quickly

c.

more, quickly

d.

more, slowly

Discuss
Answer: (c).more, quickly
Q43.
Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.

a.

DNA from collections is transcribed within in vitro and the transcription products are microinjected into oocytes

b.

The RNA is translated within oocytes

c.

Screening is carried out on the basis of DNA

d.

The screening is helpful for the proteins whose function can be easily screened such as transport of ions

Discuss
Answer: (c).Screening is carried out on the basis of DNA
Q44.
In the case of ligand binding by the expressed protein, the library can be screened by ______________

a.

In the case of ligand binding by the expressed protein, the library can be screened by ______________

b.

ligands if the sequence we are looking for encodes a protein specific to a ligand

c.

using a specific DNA sequence which can bind to the protein encoded by the sequence of interest

d.

all of the mentioned

Discuss
Answer: (d).all of the mentioned
Q45.
The loop region is single stranded. It can be cleaved by using which enzyme?

a.

Exonuclease

b.

S1 nuclease

c.

RNaseH

d.

DNase

Discuss
Answer: (b).S1 nuclease
Q46.
Choose the correct statement with respect to the self priming method of cDNA synthesis.

a.

It is less preferred than RNaseH method

b.

A hairpin structure is formed with guarantee

c.

The sequence corresponding to the 5’ end is lost

d.

Reverse transcriptase is not used

Discuss
Answer: (c).The sequence corresponding to the 5’ end is lost
Q47.
Choose the incorrect statement for the method homopolymer tailing.

a.

The first step is the RNA: DNA hybrid synthesis

b.

Terminal transferase is used for the addition of nucleotides on 3’ end

c.

Terminal transferase adds only at DNA strands

d.

The DNA strand is now having known sequence at 3’ end

Discuss
Answer: (c).Terminal transferase adds only at DNA strands
Q48.
Choose the correct statement for RACE.

a.

It stands for Random Amplification of cDNA ends

b.

It is for cloning particular cDNA ends

c.

It is only of one type, which is 5’ RACE

d.

Sequence data is not available in any case

Discuss
Answer: (b).It is for cloning particular cDNA ends
Q49.
The first primer in the case of 3’ RACE is ___________

a.

internal sequence

b.

oligo-dT adaptor molecule

c.

oligo-dA adaptor molecule

d.

adaptor oligo-dT primer

Discuss
Answer: (b).oligo-dT adaptor molecule
Q50.
The first cDNA strand in 5’ RACE is tailed with oligo-dA tail.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
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