Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Genomic and Screening Libraries. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Genomic and Screening Libraries knowledge to the test? Let's get started with our carefully curated MCQs!

Genomic and Screening Libraries MCQs | Page 7 of 12

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Q61.
Which of the following is desirable?

a.

Driver- driver hybrid

b.

Driver- tracer hybrid

c.

Tracer- tracer hybrid

d.

Driver alone

Discuss
Answer: (c).Tracer- tracer hybrid
Q62.
Which of the statement holds true?

a.

The tracer is treated with an enzyme which generates compatible ends

b.

The driver is treated with an enzyme which generates compatible ends

c.

Both driver and tracer are treated with an enzyme to generate compatible ends

d.

Both driver and tracer are treated with an enzyme to generate incompatible ends

Discuss
Answer: (a).The tracer is treated with an enzyme which generates compatible ends
Q63.
If physical methods are used, tracer is amplified and is labelled with biotin.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q64.
The process of finding a particular member of the library which is having some defined properties is called as ___________

a.

searching

b.

screening

c.

locating

d.

narrowing

Discuss
Answer: (b).screening
Q65.
If for a particular organism sequence data is available and we have to simply search in the data through a computer, then this method is called as ___________

a.

annotation

b.

database search

c.

in silico

d.

electronic search

Discuss
Answer: (c).in silico
Q66.
If we are having sequence data for a particular organism, but screening is carried out for homologues the program used is BLAST.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q67.
If screening is carried out on the basis of sequences which are related to the desired sequence, then the process is called ___________

a.

in-silico

b.

homologue search

c.

annotation

d.

partial search

Discuss
Answer: (c).annotation
Q68.
How many techniques are there for carrying out the screening of sequences encoding for RNAs?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q69.
Choose the incorrect statement for colony or plaque lift.

a.

It is the base for screening based on nucleic acid hybridization

b.

It is also known as Grunstein-Hogness technique

c.

It is based on the fact that which members of the library have the same sequence as the DNA probe

d.

The library may be plaques on a bacterial lawn and in that case it is known as plaque lift

Discuss
Answer: (c).It is based on the fact that which members of the library have the same sequence as the DNA probe
Q70.
The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps:

i) Peeling of membranes carrying away bacterial cells with it
ii) Cells are lysed and denaturing of DNA is being carried out
iii) Hybridization with labelled DNA
iv) Placing the membrane onto plate containing recombinant cells

Choose the correct sequence in which the steps are carried out (starting to ending).

a.

iv)-i)-ii)-iii)

b.

iv)-ii)-i)-iii)

c.

i)-ii)-iii)-iv)

d.

iii)-ii)-i)-iv)

Discuss
Answer: (a).iv)-i)-ii)-iii)
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