Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 9 of 12

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Q81.
At times screening is done for the protein product of DNA of interest rather than the sequence itself. How many methods are there to carry out this?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q82.
Choose the correct statement if the screening is carried out by screening by expression in vivo.

a.

The proportion of recombinants carrying gene of interest is small

b.

The recombinants carrying gene of interest don’t complement the host mutation

c.

Mutation should be affecting many genes

d.

Most of the products selected would be result of complementation

Discuss
Answer: (a).The proportion of recombinants carrying gene of interest is small
Q83.
Choose the incorrect statement with respect to host used.

a.

The host must carry mutation for the gene of interest

b.

The host should not contain restriction enzymes

c.

The host if having restriction enzymes, then the incoming DNA should not be methylated

d.

The host should be deficient in recombination

Discuss
Answer: (c).The host if having restriction enzymes, then the incoming DNA should not be methylated
Q84.
If the gene to be screened belongs to prokaryotic species then there are chances that it is not expressed.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q85.
If an expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?

a.

The extract in which the host DNA constructs are incubated is capable of translation only

b.

The extract in which the host DNA constructs are incubated is capable of transcription only

c.

The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc

d.

Non radiolabelled amino acids are used

Discuss
Answer: (c).The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc
Q86.
The direct selection allows _______ number of recombinants to be screened and _______

a.

less, slowly

b.

less, quickly

c.

more, quickly

d.

more, slowly

Discuss
Answer: (c).more, quickly
Q87.
Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.

a.

DNA from collections is transcribed within in vitro and the transcription products are microinjected into oocytes

b.

The RNA is translated within oocytes

c.

Screening is carried out on the basis of DNA

d.

The screening is helpful for the proteins whose function can be easily screened such as transport of ions

Discuss
Answer: (c).Screening is carried out on the basis of DNA
Q88.
In the case of ligand binding by the expressed protein, the library can be screened by ______________

a.

In the case of ligand binding by the expressed protein, the library can be screened by ______________

b.

ligands if the sequence we are looking for encodes a protein specific to a ligand

c.

using a specific DNA sequence which can bind to the protein encoded by the sequence of interest

d.

all of the mentioned

Discuss
Answer: (d).all of the mentioned
Q89.
If translation and transcription are taking place together, the reaction is called as ______ and if they are carried out separately, it is called as _______

a.

coupled, linked

b.

linked, coupled

c.

grouped, separate

d.

grouped, individual

Discuss
Answer: (a).coupled, linked
Q90.
If translation and transcription are carried out separately, then what is added to carry out translation?

a.

DNA polymerase

b.

RNA polymerase

c.

Taq polymerase

d.

Tfl polymerase

Discuss
Answer: (b).RNA polymerase
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