Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 8 of 12

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Q71.
For cloning purposes, the intact chromosomes should be separated by ___________

a.

agarose gel electrophoresis

b.

fluorescence- activated sorter

c.

polyacrylamide gel electrophoresis

d.

chromatography

Discuss
Answer: (b).fluorescence- activated sorter
Q72.
The process of examining stained chromosomes in a light microscope and removing appropriate regions with a micro-manipulator is called as ___________

a.

microdissection

b.

chromosome sorting

c.

chromosome walking

d.

chromosome jumping

Discuss
Answer: (a).microdissection
Q73.
If the library to be screened and the host belongs to different species, then it is called as ___________

a.

homologous selection

b.

heterologous selection

c.

intraspecies selection

d.

mixed selection

Discuss
Answer: (b).heterologous selection
Q74.
The important parameters for precise annealing of the probe and the subsequent washing don’t include ___________

a.

Size of the probe

b.

Proportion of only A

c.

Ionic concentration of hybridization buffer

d.

Other agents which alter the base pairing

Discuss
Answer: (b).Proportion of only A
Q75.
If hybridization is carried out with a clone having a sequence similar to that of the vector from which library has been constructed, then it is of no use. Choose the correct statement in respect to it.

a.

PCR amplification of the probe can be carried out

b.

Excision of the probe should be carried out which should be followed polyacrylamide gel electrophoresis

c.

If the vector sequence and the DNA probe are having similarities, some of the vector would be hybridized

d.

Probe should be in a vector that is having some sequence similarity with the library to be screened

Discuss
Answer: (a).PCR amplification of the probe can be carried out
Q76.
At what temperatures the hybridization and washing of the DNA probes should be carried out?

a.

At melting temperature

b.

At a temperature lower than the melting temperature

c.

At a temperature higher than the melting temperature

d.

At 100 degrees

Discuss
Answer: (b).At a temperature lower than the melting temperature
Q77.
Which library should be used, genomic or cDNA when the screening is carried out by oligonucleotide probes?

a.

Genomic

b.

cDNA

c.

Both genomic and cDNA library can be used equivalently

d.

None of the libraries are preferred if oligonucleotide probes are used

Discuss
Answer: (b).cDNA
Q78.
Sometimes successive rounds of screening of a genomic library are carried out and an ordered collection of clones is done in a linear fashion, then the process is called as ___________

a.

chromosome jumping

b.

chromosome sorting

c.

chromosome walking

d.

transposon tagging

Discuss
Answer: (c).chromosome walking
Q79.
If the clone for a gene is obtained on the basis of its position in the gene map, then it is called as ___________

a.

locational cloning

b.

positional cloning

c.

chromosome walking

d.

transposon tagging

Discuss
Answer: (b).positional cloning
Q80.
Mobile portions of a chromosome which can be transferred from one portion to another are termed as ___________

a.

mobile part

b.

transposons

c.

walking elements

d.

transferrable genetic element

Discuss
Answer: (b).transposons
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