Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Genomic and Screening Libraries. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Genomic and Screening Libraries knowledge to the test? Let's get started with our carefully curated MCQs!

Genomic and Screening Libraries MCQs | Page 8 of 12

Discover more Topics under Genetic Engineering

Q71.
Choose the incorrect statement with respect to the membranes used for adhering of bacterial cells onto them.

a.

Nitrocellulose membranes were preferred earlier

b.

They bind DNA very efficiently

c.

They can be handled easily without breakage

d.

They are inflammable

Discuss
Answer: (c).They can be handled easily without breakage
Q72.
Nitrocellulose membranes are less sensitive than nylon membranes.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q73.
What is used for lysing of bacterial cells and denaturation of DNA?

a.

Exonuclease

b.

Sulphuric Acid

c.

Sodium Hydroxide

d.

Heat

Discuss
Answer: (c).Sodium Hydroxide
Q74.
The process of baking or cross-linking of the membrane is carried out by ___________

a.

By heating at 80 degrees

b.

By irradiating with UV

c.

Both irradiation and heating method are used

d.

By addition of agarose

Discuss
Answer: (c).Both irradiation and heating method are used
Q75.
Choose the incorrect statement for prehybridization mixture.

a.

The function of it is to carry out specific binding

b.

It constitutes of non-labelled DNA of non-specific sequence which is obtained from salmon or herring testes or sperm

c.

It is added in a small amount

d.

It is carried out before the hybridization is carried out

Discuss
Answer: (a).The function of it is to carry out specific binding
Q76.
After hybridization how the position of the labelled DNA probe is determined?

a.

By staining

b.

By UV irradiation

c.

By using chemiluminescence

d.

By electronic microscope

Discuss
Answer: (c).By using chemiluminescence
Q77.
The important parameters for precise annealing of the probe and the subsequent washing don’t include ___________

a.

Size of the probe

b.

Proportion of only A

c.

Ionic concentration of hybridization buffer

d.

Other agents which alter the base pairing

Discuss
Answer: (b).Proportion of only A
Q78.
If hybridization is carried out with a clone having a sequence similar to that of the vector from which library has been constructed, then it is of no use. Choose the correct statement in respect to it.

a.

PCR amplification of the probe can be carried out

b.

Excision of the probe should be carried out which should be followed polyacrylamide gel electrophoresis

c.

If the vector sequence and the DNA probe are having similarities, some of the vector would be hybridized

d.

Probe should be in a vector that is having some sequence similarity with the library to be screened

Discuss
Answer: (a).PCR amplification of the probe can be carried out
Q79.
At what temperatures the hybridization and washing of the DNA probes should be carried out?

a.

At melting temperature

b.

At a temperature lower than the melting temperature

c.

At a temperature higher than the melting temperature

d.

At 100 degrees

Discuss
Answer: (b).At a temperature lower than the melting temperature
Q80.
Which library should be used, genomic or cDNA when the screening is carried out by oligonucleotide probes?

a.

Genomic

b.

cDNA

c.

Both genomic and cDNA library can be used equivalently

d.

None of the libraries are preferred if oligonucleotide probes are used

Discuss
Answer: (b).cDNA
Page 8 of 12