Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 8 of 12

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Q71.
At what temperatures the hybridization and washing of the DNA probes should be carried out?

a.

At melting temperature

b.

At a temperature lower than the melting temperature

c.

At a temperature higher than the melting temperature

d.

At 100 degrees

Discuss
Answer: (b).At a temperature lower than the melting temperature
Q72.
Which library should be used, genomic or cDNA when the screening is carried out by oligonucleotide probes?

a.

Genomic

b.

cDNA

c.

Both genomic and cDNA library can be used equivalently

d.

None of the libraries are preferred if oligonucleotide probes are used

Discuss
Answer: (b).cDNA
Q73.
Sometimes successive rounds of screening of a genomic library are carried out and an ordered collection of clones is done in a linear fashion, then the process is called as ___________

a.

chromosome jumping

b.

chromosome sorting

c.

chromosome walking

d.

transposon tagging

Discuss
Answer: (c).chromosome walking
Q74.
If the clone for a gene is obtained on the basis of its position in the gene map, then it is called as ___________

a.

locational cloning

b.

positional cloning

c.

chromosome walking

d.

transposon tagging

Discuss
Answer: (b).positional cloning
Q75.
Mobile portions of a chromosome which can be transferred from one portion to another are termed as ___________

a.

mobile part

b.

transposons

c.

walking elements

d.

transferrable genetic element

Discuss
Answer: (b).transposons
Q76.
At times screening is done for the protein product of DNA of interest rather than the sequence itself. How many methods are there to carry out this?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q77.
Choose the correct statement if the screening is carried out by screening by expression in vivo.

a.

The proportion of recombinants carrying gene of interest is small

b.

The recombinants carrying gene of interest don’t complement the host mutation

c.

Mutation should be affecting many genes

d.

Most of the products selected would be result of complementation

Discuss
Answer: (a).The proportion of recombinants carrying gene of interest is small
Q78.
Choose the incorrect statement with respect to host used.

a.

The host must carry mutation for the gene of interest

b.

The host should not contain restriction enzymes

c.

The host if having restriction enzymes, then the incoming DNA should not be methylated

d.

The host should be deficient in recombination

Discuss
Answer: (c).The host if having restriction enzymes, then the incoming DNA should not be methylated
Q79.
If the gene to be screened belongs to prokaryotic species then there are chances that it is not expressed.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q80.
If an expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?

a.

The extract in which the host DNA constructs are incubated is capable of translation only

b.

The extract in which the host DNA constructs are incubated is capable of transcription only

c.

The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc

d.

Non radiolabelled amino acids are used

Discuss
Answer: (c).The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc
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