Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 3 of 12

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Q21.
What is the basis of RNaseH method?

a.

It is based on RNA synthesis by DNA strand

b.

It is based on complementary DNA synthesis by RNA strand through reverse transcriptase

c.

It is based on complementary DNA synthesis by RNA strand through RNaseH enzyme

d.

It is based on getting double strand RNA from a single strand

Discuss
Answer: (b).It is based on complementary DNA synthesis by RNA strand through reverse transcriptase
Q22.
The first step for RNaseH method is to anneal a chemically synthesized oligo-dT primer to the 3’ polyA tail of RNA.

a.

True

b.

False

c.

May be True or False

d.

Can't Say

Discuss
Answer: (a).True
Q23.
For the synthesis of the second DNA strand, incorporated oligo-dT is of no use. Why?

a.

It is so because it is unstable

b.

It is so because it is at the 3’ end of the template molecule

c.

It is so because it would be unable to initiate replication

d.

It is so because it detaches itself after first strand synthesis

Discuss
Answer: (c).It is so because it would be unable to initiate replication
Q24.
Choose the incorrect statement for the homopolymer tailing of cDNA strands.

a.

Choose the incorrect statement for the homopolymer tailing of cDNA strands.

b.

Vector is also treated with terminal transferase and dGTPs

c.

The vector and cDNA can now anneal with the help of DNA ligase

d.

If gaps are created they can be repaired by physiological processes

Discuss
Answer: (c).The vector and cDNA can now anneal with the help of DNA ligase
Q25.
Choose the correct statement if the RNA is non polydenylated.

a.

A collection of chemically synthesized oligonucleotides is used as primers

b.

They are usually tetramer

c.

Unequal quantities of A, G, T and C are used

d.

The primers attach at only specific sequences for first strand synthesis

Discuss
Answer: (a).A collection of chemically synthesized oligonucleotides is used as primers
Q26.
Poly A tail from the column is eluted by using high salt concentration.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q27.
Choose the incorrect statement for second DNA strand synthesis.

a.

The RNA: DNA complex is treated with the enzyme RNaseH and DNA pol

b.

The enzyme RNaseH is responsible for nicking the RNA strand and leaving free 3’ hydroxyl ends

c.

These RNA fragments can be used as primers

d.

There is no portion of RNA left attached to the DNA strand

Discuss
Answer: (d).There is no portion of RNA left attached to the DNA strand
Q28.
What is the final product of the RNaseH method?

a.

blunt ended dsDNA

b.

staggered dsDNA at both ends

c.

staggered dsDNA at 3’ end

d.

staggered dsDNA at 5’ end

Discuss
Answer: (a).blunt ended dsDNA
Q29.
What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?

a.

There are no chances of the synthesis of the second DNA strand

b.

Chance complementarity would take place

c.

Hairpin structure would be formed

d.

Hairpin structure is formed is not the final structure

Discuss
Answer: (a).There are no chances of the synthesis of the second DNA strand
Q30.
The loop region is single stranded. It can be cleaved by using which enzyme?

a.

Exonuclease

b.

S1 nuclease

c.

RNaseH

d.

DNase

Discuss
Answer: (b).S1 nuclease
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