Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 3 of 12

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Q21.
Choose the incorrect statement for the preparation of genomic libraries.

a.

The first step is the isolation of genomic DNA

b.

Physical damage to the DNA should be avoided

c.

If a nuclear DNA library is to be constructed, organelle DNA is to be removed

d.

For the construction of organelle library, organelle DNA is purified from the nuclear DNA

Discuss
Answer: (c).If a nuclear DNA library is to be constructed, organelle DNA is to be removed
Q22.
The various steps for construction of libraries are ___________

i) Fragmentation of DNA
ii) Isolation of genomic DNA
iii) Amplification
iv) Ligation and introduction to the host
v) Vector preparation

The correct order of construction of libraries is (In the order of starting to ending).

a.

i)-ii)-iii)-iv)-v)

b.

ii)-i)-v)-iv)-iii)

c.

ii)-v)-i)-iv)-iii)

d.

v)-ii)-i)-iii)-iv)

Discuss
Answer: (b).ii)-i)-v)-iv)-iii)
Q23.
What is done after the recovery of pellets has been carried out in order to know the amount of polypeptides?

a.

Denaturing and gel electrophoresis in SDS- Polyacryamide gel

b.

Gel electrophoresis in agarose gel

c.

Quantitative PCR

d.

Weighing pellets

Discuss
Answer: (a).Denaturing and gel electrophoresis in SDS- Polyacryamide gel
Q24.
Sometimes a gene which we want to clone is present on a particular chromosome. For this purpose, the chromosome should be in which phase?

a.

Prophase

b.

Telophase

c.

Metaphase

d.

Anaphase

Discuss
Answer: (c).Metaphase
Q25.
For cloning purposes, the intact chromosomes should be separated by ___________

a.

agarose gel electrophoresis

b.

fluorescence- activated sorter

c.

polyacrylamide gel electrophoresis

d.

chromatography

Discuss
Answer: (b).fluorescence- activated sorter
Q26.
The process of examining stained chromosomes in a light microscope and removing appropriate regions with a micro-manipulator is called as ___________

a.

microdissection

b.

chromosome sorting

c.

chromosome walking

d.

chromosome jumping

Discuss
Answer: (a).microdissection
Q27.
If the library to be screened and the host belongs to different species, then it is called as ___________

a.

homologous selection

b.

heterologous selection

c.

intraspecies selection

d.

mixed selection

Discuss
Answer: (b).heterologous selection
Q28.
The direct selection allows _______ number of recombinants to be screened and _______

a.

less, slowly

b.

less, quickly

c.

more, quickly

d.

more, slowly

Discuss
Answer: (c).more, quickly
Q29.
Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.

a.

DNA from collections is transcribed within in vitro and the transcription products are microinjected into oocytes

b.

The RNA is translated within oocytes

c.

Screening is carried out on the basis of DNA

d.

The screening is helpful for the proteins whose function can be easily screened such as transport of ions

Discuss
Answer: (c).Screening is carried out on the basis of DNA
Q30.
In the case of ligand binding by the expressed protein, the library can be screened by ______________

a.

In the case of ligand binding by the expressed protein, the library can be screened by ______________

b.

ligands if the sequence we are looking for encodes a protein specific to a ligand

c.

using a specific DNA sequence which can bind to the protein encoded by the sequence of interest

d.

all of the mentioned

Discuss
Answer: (d).all of the mentioned
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