Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 3 of 12

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Q21.
Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.

a.

DNA from collections is transcribed within in vitro and the transcription products are microinjected into oocytes

b.

The RNA is translated within oocytes

c.

Screening is carried out on the basis of DNA

d.

The screening is helpful for the proteins whose function can be easily screened such as transport of ions

Discuss
Answer: (c).Screening is carried out on the basis of DNA
Q22.
In the case of ligand binding by the expressed protein, the library can be screened by ______________

a.

In the case of ligand binding by the expressed protein, the library can be screened by ______________

b.

ligands if the sequence we are looking for encodes a protein specific to a ligand

c.

using a specific DNA sequence which can bind to the protein encoded by the sequence of interest

d.

all of the mentioned

Discuss
Answer: (d).all of the mentioned
Q23.
In the case of immunochemical screening, the position of ________ antibody is detected by ________ antibody.

a.

secondary, primary

b.

primary, secondary

c.

primary, tertiary

d.

secondary, tertiary

Discuss
Answer: (b).primary, secondary
Q24.
The ligand should bind to a single protein only for the screening process.

a.

True

b.

False

c.

May be True or False

d.

Can't Say

Discuss
Answer: (a).True
Q25.
Choose the incorrect statement for panning.

a.

It is a variant of ligand binding approach

b.

The ligand is immobilized on the solid support and the cells are passed over it

c.

If the ligand-binding domain is exposed on surface of the any recombinant molecules then they will bind to ligand

d.

They are not useful for scanning libraries in cultured mammalian cells

Discuss
Answer: (d).They are not useful for scanning libraries in cultured mammalian cells
Q26.
If screening is carried out by using a combination of nascent peptide and mRNA, then it is called as ________

a.

nascent peptide display

b.

mRNA display

c.

ribosome display

d.

ribozyme display

Discuss
Answer: (c).ribosome display
Q27.
Libraries can broadly be classified into how many types?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q28.
Choose the correct statement for genomic libraries.

a.

Genomic libraries include the representation of the whole genome of the organism

b.

Sequences such as telomeres are also represented

c.

Telomeres can be readily cloned

d.

The larger the size of the insert of genomic DNA in recombinants, the more is the number of recombinants required to represent the genome in the library

Discuss
Answer: (a).Genomic libraries include the representation of the whole genome of the organism
Q29.
Choose the incorrect statement for the preparation of genomic libraries.

a.

The first step is the isolation of genomic DNA

b.

Physical damage to the DNA should be avoided

c.

If a nuclear DNA library is to be constructed, organelle DNA is to be removed

d.

For the construction of organelle library, organelle DNA is purified from the nuclear DNA

Discuss
Answer: (c).If a nuclear DNA library is to be constructed, organelle DNA is to be removed
Q30.
The various steps for construction of libraries are ___________

i) Fragmentation of DNA
ii) Isolation of genomic DNA
iii) Amplification
iv) Ligation and introduction to the host
v) Vector preparation

The correct order of construction of libraries is (In the order of starting to ending).

a.

i)-ii)-iii)-iv)-v)

b.

ii)-i)-v)-iv)-iii)

c.

ii)-v)-i)-iv)-iii)

d.

v)-ii)-i)-iii)-iv)

Discuss
Answer: (b).ii)-i)-v)-iv)-iii)
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