Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 10 of 12

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Q91.
GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.

a.

It stands for Green Fluorescent Protein

b.

It is obtained from a bio-luminescent jellyfish and produces protein aequorin which emits blue light

c.

The blue light is produced because of binding of sodium ions

d.

The absorbed blue light produces green light which can be detected further

Discuss
Answer: (c).The blue light is produced because of binding of sodium ions
Q92.
Luciferase genes are also used at times for detection. Choose the correct statement for them.

a.

They are obtained from fire flies only

b.

The detection requires provision of substrate which produces light

c.

Enzymes such as beta-galactosidase requires substrate X-gluc to produce light

d.

Lucifearse genes are preferred over fluorescent proteins

Discuss
Answer: (b).The detection requires provision of substrate which produces light
Q93.
In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q94.
Sequences that can function as origins of replication are called as ___________

a.

partial replicating sequences

b.

self replicating sequences

c.

autonomously replicating sequences

d.

modified replicating sequences

Discuss
Answer: (c).autonomously replicating sequences
Q95.
Screening is often carried out for a sequence that interacts with a protein for which already a clone is present. It is carried out in which host?

a.

Bacterial host

b.

Fungal host

c.

Parasitic host

d.

Yeast host

Discuss
Answer: (d).Yeast host
Q96.
In two hybrid screening system, the activator binds through _______ domain to a sequence upstream of the gene under its control, and ________ domain stimulates transcription.

a.

DNA binding, activation

b.

Activation, DNA binding

c.

Activation, transcription

d.

DNA binding, transcription

Discuss
Answer: (a).DNA binding, activation
Q97.
For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein.

a.

prey

b.

bait

c.

binding

d.

activation

Discuss
Answer: (b).bait
Q98.
What is done after the recovery of pellets has been carried out in order to know the amount of polypeptides?

a.

Denaturing and gel electrophoresis in SDS- Polyacryamide gel

b.

Gel electrophoresis in agarose gel

c.

Quantitative PCR

d.

Weighing pellets

Discuss
Answer: (a).Denaturing and gel electrophoresis in SDS- Polyacryamide gel
Q99.
Sometimes a gene which we want to clone is present on a particular chromosome. For this purpose, the chromosome should be in which phase?

a.

Prophase

b.

Telophase

c.

Metaphase

d.

Anaphase

Discuss
Answer: (c).Metaphase
Q100.
For cloning purposes, the intact chromosomes should be separated by ___________

a.

agarose gel electrophoresis

b.

fluorescence- activated sorter

c.

polyacrylamide gel electrophoresis

d.

chromatography

Discuss
Answer: (b).fluorescence- activated sorter
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