Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 10 of 12

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Q91.
If the screening of the DNA constructs is carried out in pooled batches then it takes less time.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q92.
Choose the incorrect statement for the open reading frame.

a.

It is helpful in deciding whether the clone is correct or not

b.

It consists of an initiation codon

c.

It consists of a termination codon

d.

The presence of introns doesn’t affect the open reading frame

Discuss
Answer: (d).The presence of introns doesn’t affect the open reading frame
Q93.
Introns are ________ in nature and the sequence is _______ than exons.

a.

coding, less random

b.

non-coding, less random

c.

non-coding, more random

d.

coding, more random

Discuss
Answer: (c).non-coding, more random
Q94.
If the genome size is small, then sequencing of the entire genome is ___________

a.

easy

b.

difficult

c.

independent of the size of genome

d.

not possible

Discuss
Answer: (a).easy
Q95.
Choose the correct statement with respect to the bacterial genome.

a.

They are easy to sequence

b.

They are difficult to sequence

c.

Methods such as transcription and translation are satisfactory

d.

Only transcription can be carried out

Discuss
Answer: (b).They are difficult to sequence
Q96.
For identification of genes that are expressed under special conditions, _______ libraries are used.

a.

cDNA

b.

genomic

c.

subtractive

d.

shelf

Discuss
Answer: (c).subtractive
Q97.
In ______ organisms, firstly the gene is looked into model organisms.

a.

eukaryotic

b.

prokaryotic

c.

both eukaryotic and prokaryotic

d.

large sized

Discuss
Answer: (a).eukaryotic
Q98.
Promoter-probe vectors are used often. Choose the correct statement for these vectors.

a.

They are used for identifying sequences that can function as promoter in vivo

b.

It contains a reporter gene which contains its promoter along

c.

The reporter gene is having a cloning site

d.

After insertion of DNA into the cloning site, selection of plasmids is carried out by blue white screening

Discuss
Answer: (a).They are used for identifying sequences that can function as promoter in vivo
Q99.
GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.

a.

It stands for Green Fluorescent Protein

b.

It is obtained from a bio-luminescent jellyfish and produces protein aequorin which emits blue light

c.

The blue light is produced because of binding of sodium ions

d.

The absorbed blue light produces green light which can be detected further

Discuss
Answer: (c).The blue light is produced because of binding of sodium ions
Q100.
Luciferase genes are also used at times for detection. Choose the correct statement for them.

a.

They are obtained from fire flies only

b.

The detection requires provision of substrate which produces light

c.

Enzymes such as beta-galactosidase requires substrate X-gluc to produce light

d.

Lucifearse genes are preferred over fluorescent proteins

Discuss
Answer: (b).The detection requires provision of substrate which produces light
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