Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 6 of 12

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Q51.
What is the second primer in the case of 5’ RACE?

a.

Internal primer

b.

Oligo-dA sequence

c.

Adaptor-oligo-dT primer

d.

Oligo-dT adaptor molecule

Discuss
Answer: (c).Adaptor-oligo-dT primer
Q52.
RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

a.

Blunt ended ligation

b.

Addition of linkers

c.

Using appropriate restriction enzymes

d.

All the methods can be used equivalently

Discuss
Answer: (d).All the methods can be used equivalently
Q53.
Choose the correct statement for modification of homopolymer tailing method.

a.

It includes modification of primers

b.

Primers are varied by simply altering their size by randomly adding or removing bases

c.

The 5’ end of the first cDNA strand is tailed with C residues

d.

A single stranded oilgonucleotide is then used for second strand synthesis

Discuss
Answer: (a).It includes modification of primers
Q54.
By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q55.
Sometimes the required mRNA is present in less number. So the process of increasing the representation of rare mRNAs is called as ____________

a.

amplification

b.

normalization

c.

selection

d.

narrowing

Discuss
Answer: (b).normalization
Q56.
Choose the correct statement in order to enrich rare species.

a.

The most basic principle relies on the kinetics of hybridization

b.

A collection of single stranded molecules is allowed to reanneal under given conditions

c.

The strands which are less abundant are able to find their complement easily

d.

The left over molecules are more abundant in nature

Discuss
Answer: (a).The most basic principle relies on the kinetics of hybridization
Q57.
Which statement holds true for hydroxyapatite?

a.

It binds to single stranded molecules more tightly than double stranded molecules

b.

It binds to linear molecules more tightly than circular molecules

c.

It binds to circular molecules more tightly than linear molecules

d.

It binds to double stranded molecules more tightly than single stranded molecules

Discuss
Answer: (d).It binds to double stranded molecules more tightly than single stranded molecules
Q58.
Libraries in which a particular sequence is present in one organism but are absent from another organism, are called as ___________

a.

normalized libraries

b.

subtractive libraries

c.

selective libraries

d.

partial libraries

Discuss
Answer: (b).subtractive libraries
Q59.
The nucleic acid from the cell type that contains the sequence we are interested in is called ___________

a.

driver

b.

subtractive sequence

c.

tracer

d.

wanted sequence

Discuss
Answer: (c).tracer
Q60.
Choose the correct statement for libraries constructed by mixing cell types differing in the sequences they are having.

a.

The driver and tracer are mixed in double stranded form

b.

Tracer is in stoichiometric excess in comparison to driver

c.

Tracer and driver are in the same amount

d.

The sequences are allowed to hybridize

Discuss
Answer: (d).The sequences are allowed to hybridize
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