Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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Genomic and Screening Libraries MCQs | Page 2 of 12

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Q11.
Choose the incorrect statement for the homopolymer tailing of cDNA strands.

a.

Choose the incorrect statement for the homopolymer tailing of cDNA strands.

b.

Vector is also treated with terminal transferase and dGTPs

c.

The vector and cDNA can now anneal with the help of DNA ligase

d.

If gaps are created they can be repaired by physiological processes

Discuss
Answer: (c).The vector and cDNA can now anneal with the help of DNA ligase
Q12.
Choose the correct statement if the RNA is non polydenylated.

a.

A collection of chemically synthesized oligonucleotides is used as primers

b.

They are usually tetramer

c.

Unequal quantities of A, G, T and C are used

d.

The primers attach at only specific sequences for first strand synthesis

Discuss
Answer: (a).A collection of chemically synthesized oligonucleotides is used as primers
Q13.
Choose the correct statement for genomic libraries.

a.

Genomic libraries include the representation of the whole genome of the organism

b.

Sequences such as telomeres are also represented

c.

Telomeres can be readily cloned

d.

The larger the size of the insert of genomic DNA in recombinants, the more is the number of recombinants required to represent the genome in the library

Discuss
Answer: (a).Genomic libraries include the representation of the whole genome of the organism
Q14.
Choose the incorrect statement for the preparation of genomic libraries.

a.

The first step is the isolation of genomic DNA

b.

Physical damage to the DNA should be avoided

c.

If a nuclear DNA library is to be constructed, organelle DNA is to be removed

d.

For the construction of organelle library, organelle DNA is purified from the nuclear DNA

Discuss
Answer: (c).If a nuclear DNA library is to be constructed, organelle DNA is to be removed
Q15.
The various steps for construction of libraries are ___________

i) Fragmentation of DNA
ii) Isolation of genomic DNA
iii) Amplification
iv) Ligation and introduction to the host
v) Vector preparation

The correct order of construction of libraries is (In the order of starting to ending).

a.

i)-ii)-iii)-iv)-v)

b.

ii)-i)-v)-iv)-iii)

c.

ii)-v)-i)-iv)-iii)

d.

v)-ii)-i)-iii)-iv)

Discuss
Answer: (b).ii)-i)-v)-iv)-iii)
Q16.
In case if molecules smaller than the fragments required for making a full genomic library are used for making a collection. This collection is called as ___________

a.

library

b.

shelf

c.

small library

d.

mini library

Discuss
Answer: (b).shelf
Q17.
Choose the correct statement for construction of a library subsection.

a.

The size of a particular restriction fragment on which the gene is located is not known

b.

The size of the restriction fragment can be known by carrying out southern blotting

c.

Another digest of the genomic DNA is carried out by a different enzyme

d.

DNA fragments of different size are recovered after carrying out gel electrophoresis

Discuss
Answer: (b).The size of the restriction fragment can be known by carrying out southern blotting
Q18.
Any cDNA library would represent a fraction of RNA species of an organism.

a.

True

b.

False

c.

May be True or False

d.

Can't Say

Discuss
Answer: (a).True
Q19.
What do we mean by housekeeping genes?

a.

Housekeeping genes are those genes which are specific to an organism

b.

Housekeeping genes are those genes which are present in all the organisms

c.

Housekeeping genes are those genes which are meant for repair and maintenance in a species of organism

d.

Housekeeping genes are those genes which required for the replication process

Discuss
Answer: (b).Housekeeping genes are those genes which are present in all the organisms
Q20.
Choose the correct statement for RNA fractionation.

a.

The RNA is fractioned by size but before separating on oligo-dT cellulose

b.

A sucrose density gradient is used

c.

The RNA is applied to the top of a pre-poured gradient and during centrifugation smaller molecules move down the tube faster

d.

Different bands are formed according to the density in the sucrose density gradient

Discuss
Answer: (b).A sucrose density gradient is used
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