Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

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So, are you ready to put your Genomic and Screening Libraries knowledge to the test? Let's get started with our carefully curated MCQs!

Genomic and Screening Libraries MCQs | Page 2 of 12

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Q11.
What do we mean by housekeeping genes?

a.

Housekeeping genes are those genes which are specific to an organism

b.

Housekeeping genes are those genes which are present in all the organisms

c.

Housekeeping genes are those genes which are meant for repair and maintenance in a species of organism

d.

Housekeeping genes are those genes which required for the replication process

Discuss
Answer: (b).Housekeeping genes are those genes which are present in all the organisms
Q12.
Choose the correct statement for RNA fractionation.

a.

The RNA is fractioned by size but before separating on oligo-dT cellulose

b.

A sucrose density gradient is used

c.

The RNA is applied to the top of a pre-poured gradient and during centrifugation smaller molecules move down the tube faster

d.

Different bands are formed according to the density in the sucrose density gradient

Discuss
Answer: (b).A sucrose density gradient is used
Q13.
What is done after RNA fractionation is carried out?

a.

Each band is translated in vivo

b.

Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells

c.

Translation is carried out with a high background

d.

Amino acid is not radioactively labelled

Discuss
Answer: (b).Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells
Q14.
The polypeptides produced after addition of mRNA are analysed with antibodies. Choose the incorrect statement for this analysis.

a.

Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction

b.

Along with simple antigen-antibody complex, a substrate is added for easy precipitation

c.

Protein A-Sepharose is added

d.

Protein A-Sepharose binds to IgG antibodies

Discuss
Answer: (a).Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction
Q15.
In the case of immunochemical screening, the position of ________ antibody is detected by ________ antibody.

a.

secondary, primary

b.

primary, secondary

c.

primary, tertiary

d.

secondary, tertiary

Discuss
Answer: (b).primary, secondary
Q16.
The ligand should bind to a single protein only for the screening process.

a.

True

b.

False

c.

May be True or False

d.

Can't Say

Discuss
Answer: (a).True
Q17.
Choose the incorrect statement for panning.

a.

It is a variant of ligand binding approach

b.

The ligand is immobilized on the solid support and the cells are passed over it

c.

If the ligand-binding domain is exposed on surface of the any recombinant molecules then they will bind to ligand

d.

They are not useful for scanning libraries in cultured mammalian cells

Discuss
Answer: (d).They are not useful for scanning libraries in cultured mammalian cells
Q18.
If screening is carried out by using a combination of nascent peptide and mRNA, then it is called as ________

a.

nascent peptide display

b.

mRNA display

c.

ribosome display

d.

ribozyme display

Discuss
Answer: (c).ribosome display
Q19.
Libraries can broadly be classified into how many types?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q20.
What is done after the recovery of pellets has been carried out in order to know the amount of polypeptides?

a.

Denaturing and gel electrophoresis in SDS- Polyacryamide gel

b.

Gel electrophoresis in agarose gel

c.

Quantitative PCR

d.

Weighing pellets

Discuss
Answer: (a).Denaturing and gel electrophoresis in SDS- Polyacryamide gel
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