Genomic and Screening Libraries MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Genomic and Screening Libraries, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Genomic and Screening Libraries MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Genomic and Screening Libraries mcq questions that explore various aspects of Genomic and Screening Libraries problems. Each MCQ is crafted to challenge your understanding of Genomic and Screening Libraries principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Genomic and Screening Libraries MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Genomic and Screening Libraries. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Genomic and Screening Libraries knowledge to the test? Let's get started with our carefully curated MCQs!

Genomic and Screening Libraries MCQs | Page 2 of 12

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Q11.
What do we mean by housekeeping genes?

a.

Housekeeping genes are those genes which are specific to an organism

b.

Housekeeping genes are those genes which are present in all the organisms

c.

Housekeeping genes are those genes which are meant for repair and maintenance in a species of organism

d.

Housekeeping genes are those genes which required for the replication process

Discuss
Answer: (b).Housekeeping genes are those genes which are present in all the organisms
Q12.
Choose the correct statement for RNA fractionation.

a.

The RNA is fractioned by size but before separating on oligo-dT cellulose

b.

A sucrose density gradient is used

c.

The RNA is applied to the top of a pre-poured gradient and during centrifugation smaller molecules move down the tube faster

d.

Different bands are formed according to the density in the sucrose density gradient

Discuss
Answer: (b).A sucrose density gradient is used
Q13.
What is done after RNA fractionation is carried out?

a.

Each band is translated in vivo

b.

Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells

c.

Translation is carried out with a high background

d.

Amino acid is not radioactively labelled

Discuss
Answer: (b).Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells
Q14.
The polypeptides produced after addition of mRNA are analysed with antibodies. Choose the incorrect statement for this analysis.

a.

Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction

b.

Along with simple antigen-antibody complex, a substrate is added for easy precipitation

c.

Protein A-Sepharose is added

d.

Protein A-Sepharose binds to IgG antibodies

Discuss
Answer: (a).Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction
Q15.
What is done after the recovery of pellets has been carried out in order to know the amount of polypeptides?

a.

Denaturing and gel electrophoresis in SDS- Polyacryamide gel

b.

Gel electrophoresis in agarose gel

c.

Quantitative PCR

d.

Weighing pellets

Discuss
Answer: (a).Denaturing and gel electrophoresis in SDS- Polyacryamide gel
Q16.
Sometimes a gene which we want to clone is present on a particular chromosome. For this purpose, the chromosome should be in which phase?

a.

Prophase

b.

Telophase

c.

Metaphase

d.

Anaphase

Discuss
Answer: (c).Metaphase
Q17.
For cloning purposes, the intact chromosomes should be separated by ___________

a.

agarose gel electrophoresis

b.

fluorescence- activated sorter

c.

polyacrylamide gel electrophoresis

d.

chromatography

Discuss
Answer: (b).fluorescence- activated sorter
Q18.
The process of examining stained chromosomes in a light microscope and removing appropriate regions with a micro-manipulator is called as ___________

a.

microdissection

b.

chromosome sorting

c.

chromosome walking

d.

chromosome jumping

Discuss
Answer: (a).microdissection
Q19.
If the library to be screened and the host belongs to different species, then it is called as ___________

a.

homologous selection

b.

heterologous selection

c.

intraspecies selection

d.

mixed selection

Discuss
Answer: (b).heterologous selection
Q20.
The direct selection allows _______ number of recombinants to be screened and _______

a.

less, slowly

b.

less, quickly

c.

more, quickly

d.

more, slowly

Discuss
Answer: (c).more, quickly
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