Vector Systems MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Vector Systems, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Vector Systems MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Vector Systems mcq questions that explore various aspects of Vector Systems problems. Each MCQ is crafted to challenge your understanding of Vector Systems principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Vector Systems MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Vector Systems. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Vector Systems knowledge to the test? Let's get started with our carefully curated MCQs!

Vector Systems MCQs | Page 1 of 9

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Q1.
At times, a second enzyme is used while the central portion is removed and the stuffer fragment is placed there. Choose the statement which doesn’t holds for this process.

a.

It is a method which is used for avoiding the relegation of central portion before the stuffer fragment is attached over there

b.

In this method, the central portion is cut with another enzyme after being separated from the arms

c.

The arms are also cut with this second enzyme

d.

It is not necessary to remove the fragments generated

Discuss
Answer: (c).The arms are also cut with this second enzyme
Q2.
Physical separation is also used at times. Choose the correct statement with respect to this method.

a.

Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient

b.

In sugar density gradient, the molecules are separated on the basis of density

c.

The central portion is recovered from the gel or the gradient

d.

Gel is more efficient than the sugar density gradient

Discuss
Answer: (a).Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient
Q3.
Packaging in vitro is also carried out to introduce the lambda DNA into the cells. Choose the incorrect statement in respect to it.

a.

Packaging in phage coat is a more efficient process than the normal transformation process

b.

The lambda DNA is incubated with lysate of lambda infected cells in a concatemeric configuration

c.

Lysate constitutes of lambda proteins needed for phage assembly

d.

The amount of non-recombinant phage in the background is less

Discuss
Answer: (d).The amount of non-recombinant phage in the background is less
Q4.
Packaging in vitro is basically carried out by two different strategies. Choose the incorrect statement for it.

a.

Separate strains with chain-termination mutations in different genes for coat components are used

b.

Temperature sensitive cI repressor is used

c.

Due to heat shock packaging proteins are induced

d.

Packaging is carried out in each strain

Discuss
Answer: (d).Packaging is carried out in each strain
Q5.
Chain-termination mutations in coat proteins genes are carried out. The coat protein coat genes used are __________

a.

A gene

b.

D gene

c.

E and D gene

d.

D and A gene

Discuss
Answer: (c).E and D gene
Q6.
Which of the following is a restriction for cloning in bacteriophage Mu?

a.

It is limited to handling only small number of phage particles

b.

It relies on whether the DNA is flanked by two mini Mu genes or not

c.

Recombination between the elements in the second strain is not a restriction

d.

Fragments of any genomic DNA can be cloned irrespective of the fact whether or not the phage can replicate in it or not

Discuss
Answer: (b).It relies on whether the DNA is flanked by two mini Mu genes or not
Q7.
What is the size of the insert that can be accommodated in the head of bacteriophage PI?

a.

30-40 kbp

b.

80-95 kbp

c.

110-115 kbp

d.

200-300 kbp

Discuss
Answer: (c).110-115 kbp
Q8.
What is the function of the pac site in the bacteriophage PI?

a.

It is responsible for the initiation of packaging

b.

It is responsible for the termination of packaging

c.

It is responsible for the initiation of replication

d.

It is responsible for the termination of replication

Discuss
Answer: (a).It is responsible for the initiation of packaging
Q9.
What is the function of loxP sites?

a.

It is responsible for linearization of DNA in the host bacterium

b.

It is responsible for circularization of DNA in the host bacterium

c.

It is responsible for conversion of single stranded DNA into double stranded DNA

d.

It is responsible for conversion of double stranded DNA into single stranded DNA

Discuss
Answer: (b).It is responsible for circularization of DNA in the host bacterium
Q10.
Which of the following is not included as a step in cloning in bacteriophage PI?

a.

The growth of vector is done as a plasmid by using pBR322 origin of replication

b.

Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated

c.

Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites

d.

Incubation is done with mutant lysogen which doesn’t contains head and tail proteins

Discuss
Answer: (c).Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites
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