Vector Systems MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Vector Systems, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Vector Systems MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Vector Systems mcq questions that explore various aspects of Vector Systems problems. Each MCQ is crafted to challenge your understanding of Vector Systems principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Vector Systems MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Vector Systems. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Vector Systems knowledge to the test? Let's get started with our carefully curated MCQs!

Vector Systems MCQs | Page 2 of 9

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Q11.
What happens after packaging is carried out in the phage head?

a.

A sequence dependent cleavage of DNA is carried out

b.

A sequence independent cleavage of DNA is carried out

c.

They are linearized before they are infected into the bacterial cells

d.

They are circularized before they are infected into the bacterial cells

Discuss
Answer: (b).A sequence independent cleavage of DNA is carried out
Q12.
Circularization must take place in the bacterial cells before the replication starts.

a.

True

b.

False

c.

May be True or False

d.

Can't Say

Discuss
Answer: (a).True
Q13.
How many origins of replication are there once the cyclization is carried out by loxP sites?

a.

1

b.

2

c.

3

d.

4

Discuss
Answer: (b).2
Q14.
Choose the incorrect statement for PAC vectors.

a.

In these vectors, the phage packaging signals are removed but the two P1 origin of replication persist

b.

They resemble BAC vectors

c.

They accept an insert of upto 300 kbp

d.

Instability is observed

Discuss
Answer: (d).Instability is observed
Q15.
Choose the correct statement in respect to sacB gene?

a.

The cloning site for PAC lies on the side of sacB gene

b.

It is responsible for producing an enzyme which is responsible for catalysing sucrose into glucose and fructose

c.

Expression of sacB in the presence of sucrose is beneficial

d.

Levan is non-toxic for E. coli

Discuss
Answer: (b).It is responsible for producing an enzyme which is responsible for catalysing sucrose into glucose and fructose
Q16.
Selection of molecules by lack of inserts on the basis of the sacB gene is known as:

a.

positive selection

b.

negative selection

c.

counter selection

d.

sacB selection

Discuss
Answer: (c).counter selection
Q17.
F (fertility) factor is used in many bacterial systems for different purposes. Choose the incorrect statement with respect to the F factor.

a.

It was identified in E. coli for catalysing genetic exchange between cells

b.

Cells having F factor are called as female

c.

The F factor is transferred from one cell to another via factors present outside

d.

They are not related to BAC systems

Discuss
Answer: (a).It was identified in E. coli for catalysing genetic exchange between cells
Q18.
F factor integration into the host chromosome is called as __________

a.

F’ plasmid

b.

F factor and host chromosome recombination

c.

Hfr (High frequency of recombination)

d.

Recombinant host chromosome

Discuss
Answer: (c).Hfr (High frequency of recombination)
Q19.
Excision of the F factor can also be done from the host chromosome. Choose the incorrect statement in respect to it.

a.

This excision is possible in vivo

b.

Along with the F factor, host chromosome’s DNA sequences are also excited

c.

The sequences of host chromosome which can be accommodated in the F plasmid are small in size

d.

These plasmids are termed as F’ plasmids

Discuss
Answer: (c).The sequences of host chromosome which can be accommodated in the F plasmid are small in size
Q20.
The low copy number of F plasmid is essential for the stability of these plasmids.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
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