Vector Systems MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Vector Systems, a fundamental topic in the field of Genetic Engineering. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Vector Systems MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Vector Systems mcq questions that explore various aspects of Vector Systems problems. Each MCQ is crafted to challenge your understanding of Vector Systems principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Genetic Engineering tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Vector Systems MCQs are your pathway to success in mastering this essential Genetic Engineering topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of Vector Systems. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your Vector Systems knowledge to the test? Let's get started with our carefully curated MCQs!

Vector Systems MCQs | Page 2 of 9

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Q11.
What is the size of the DNA molecule which can be packed by the viral protein coat?

a.

100kbp

b.

200kbp

c.

500kbp

d.

No size restriction

Discuss
Answer: (d).No size restriction
Q12.
The uniform layer of cells is known as lawn and the holes peppered from infected cells are termed as __________

a.

plaques

b.

holes

c.

colony

d.

pothole

Discuss
Answer: (a).plaques
Q13.
M13 doesn’t actually lyse the cells.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q14.
The plaques formed by M13 infection are called as __________

a.

retarded plaques

b.

true plaques

c.

pseudoplaques

d.

lysogenized plaques

Discuss
Answer: (c).pseudoplaques
Q15.
M13 vector is tightly packed. Where is the available site to insert DNA?

a.

In the gene II

b.

Between gene II and IV

c.

Between gene III and V

d.

Between gene V and VIII

Discuss
Answer: (b).Between gene II and IV
Q16.
Choose the incorrect statement with respect to the collection of single stranded molecules.

a.

An infected culture is set up and after a while the cells are removed by centrifugation

b.

The phage is in the supernatant and is precipitated by addition of glycol and sodium chloride

c.

The phage pellet is resuspended and phenol is used for removal of protein

d.

The single stranded molecules sediment at the bottom

Discuss
Answer: (d).The single stranded molecules sediment at the bottom
Q17.
At times, a second enzyme is used while the central portion is removed and the stuffer fragment is placed there. Choose the statement which doesn’t holds for this process.

a.

It is a method which is used for avoiding the relegation of central portion before the stuffer fragment is attached over there

b.

In this method, the central portion is cut with another enzyme after being separated from the arms

c.

The arms are also cut with this second enzyme

d.

It is not necessary to remove the fragments generated

Discuss
Answer: (c).The arms are also cut with this second enzyme
Q18.
Physical separation is also used at times. Choose the correct statement with respect to this method.

a.

Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient

b.

In sugar density gradient, the molecules are separated on the basis of density

c.

The central portion is recovered from the gel or the gradient

d.

Gel is more efficient than the sugar density gradient

Discuss
Answer: (a).Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient
Q19.
Packaging in vitro is also carried out to introduce the lambda DNA into the cells. Choose the incorrect statement in respect to it.

a.

Packaging in phage coat is a more efficient process than the normal transformation process

b.

The lambda DNA is incubated with lysate of lambda infected cells in a concatemeric configuration

c.

Lysate constitutes of lambda proteins needed for phage assembly

d.

The amount of non-recombinant phage in the background is less

Discuss
Answer: (d).The amount of non-recombinant phage in the background is less
Q20.
Packaging in vitro is basically carried out by two different strategies. Choose the incorrect statement for it.

a.

Separate strains with chain-termination mutations in different genes for coat components are used

b.

Temperature sensitive cI repressor is used

c.

Due to heat shock packaging proteins are induced

d.

Packaging is carried out in each strain

Discuss
Answer: (d).Packaging is carried out in each strain
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